Curcumin–galactomannoside complex inhibits pathogenesis in Ox-LDL-challenged human peripheral blood mononuclear cells

Saji, S and Asha, S and Svenia, PJ and Ratheesh, M and Sheethal, S and Sandya, S and Krishnakumar, IM (2018) Curcumin–galactomannoside complex inhibits pathogenesis in Ox-LDL-challenged human peripheral blood mononuclear cells. In: Inflammopharmacology, 26 (5). pp. 1273-1282.

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Abstract

Oxidised low-density lipoprotein (ox-LDL) is a pro-atherogenic molecule, which induces inflammatory response and contributes to the pathogenesis of vascular dysfunction to atherosclerosis. The aim of the present study was to explore the anti-inflammatory effect of a novel bioavailable formulation of curcumin as ‘curcumagalactomannosides’ (CGM) against ox-LDL-induced inflammatory responses in human peripheral blood mononuclear cells (hPBMCs). Curcumagalactomannosides was made from natural curcumin using the soluble dietary fibre (galactomannans) derived from fenugreek seeds (Trigonella foenumgracum) and the hPBMCs were isolated from healthy human volunteers. The cells were cultured in collagen-coated plates at 37 °C and grouped as Group I (Control), Group II (ox-LDL treated) and Group III (ox-LDL + CGM treated). Further analysis of inflammatory markers, reactive oxygen species and mRNA expression levels indicated significantly increased expressions of iNOS, TNF-α, IL-6 and VCAM-1 in ox-LDL-treated group along with the nuclear translocation of NF-κB. Other inflammatory markers such as LOX, PGE2, total COX and lipid peroxidation level were also found to be significantly (p < 0.05) increased upon ox-LDL treatment. The treatment with CGM on the other hand was found to down-regulate and reverse the ox-LDL-induced alterations indicating its potential anti-inflammatory effect on hPBMCs via. NF-κB signalling pathway.

Item Type:	Journal Article
Publication:	Inflammopharmacology
Publisher:	Birkhauser Verlag AG
Additional Information:	The copyright for this article belongs to the Birkhauser Verlag AG.
Keywords:	collagen type 1; curcumin; galactomannan; immunoglobulin enhancer binding protein; inducible nitric oxide synthase; interleukin 6; lipoxygenase; mannoside; messenger RNA; oxidized low density lipoprotein; prostaglandin E2; prostaglandin synthase; reactive oxygen metabolite; thiobarbituric acid reactive substance; tumor necrosis factor; vascular cell adhesion molecule 1; curcumin; galactoside; immunoglobulin enhancer binding protein; low density lipoprotein; mannoside; oxidized low density lipoprotein; reactive oxygen metabolite; tumor necrosis factor; vascular cell adhesion molecule 1, antiinflammatory activity; Article; cell isolation; cell viability; complex formation; controlled study; dietary fiber; down regulation; drug cytotoxicity; enzyme linked immunosorbent assay; fenugreek; human; human cell; human cell culture; inflammation; lipid peroxidation; mRNA expression level; MTT assay; pathogenesis; peripheral blood mononuclear cell; plant seed; priority journal; protein localization; reverse transcription polymerase chain reaction; signal transduction; Trigonella; Trigonella foenumgracum; upregulation; cell culture; cell survival; drug effect; genetics; metabolism; mononuclear cell; physiology, Cell Survival; Cells, Cultured; Curcumin; Galactosides; Humans; Leukocytes, Mononuclear; Lipid Peroxidation; Lipoproteins, LDL; Mannosides; NF-kappa B; Reactive Oxygen Species; Tumor Necrosis Factor-alpha; Vascular Cell Adhesion Molecule-1
Department/Centre:	Division of Chemical Sciences > Inorganic & Physical Chemistry
Date Deposited:	05 Aug 2022 09:34
Last Modified:	05 Aug 2022 09:34
URI:	https://eprints.iisc.ac.in/id/eprint/75365

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