Shashikumar, R and Krishna, V and Venkatesh, Venkatesh and Lokesha, S (2017) Analysis of genetic variations in musa paradisiaca cv karibale-monthan using aflp markers. In: Indian Journal of Agricultural Research, 51 (6). pp. 543-549.
Full text not available from this repository.Abstract
In the present study, the genetic variations of leaf calli regenerants of Musa paradisiaca cv Karibale-Monthan has been analyzed using AFLP markers. The highest (86.66%) frequency of compact callus formation was obtained when leaf explants were cultured on MS media, containing 3mg/L 2,4-D and 0.5 mg/L BAP. The optimum shoot formation and multiplication (5.70±1.49) was achieved from the leaf derived callus on MS medium fortified with 3mg/L BAP+0.5mg/L TDZ. The 3-4 cm small shoots produced distinct roots on MS media containing 0.5mg/l NAA with 0.2% activated charcoal. The AFLP analyses produced 1012 monomorphic bands from total 1094 bands with 7.5% polymorphism between mother plant and callus derived regenerants. The percentage of monomorphism for individual primer combinations was very high and varied from 90.3% (EcoRI-AG×MseI-CCG) to 97.6% (EcoRI-AT×MseI-CGA). The result showed lack of polymorphism is due to genetic integrity of the plants that was not altered indifferent to the hormonal treatment.
| Item Type: | Journal Article |
|---|---|
| Publication: | Indian Journal of Agricultural Research |
| Publisher: | Agricultural Research Communication Centre |
| Additional Information: | The copyright for this article belongs to the Agricultural Research Communication Centre. |
| Department/Centre: | Division of Biological Sciences > Biochemistry |
| Date Deposited: | 15 Jul 2022 11:24 |
| Last Modified: | 15 Jul 2022 11:24 |
| URI: | https://eprints.iisc.ac.in/id/eprint/74452 |
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