Sub 100 nm resolution confocal focus-engineered coherent anti-Stokes Raman scattering microscopy under non-degenerate pumping condition

Lee, G and Jyothsna, KM and Lim, H and Park, J and Lee, J and Raghunathan, V and Kim, H (2022) Sub 100 nm resolution confocal focus-engineered coherent anti-Stokes Raman scattering microscopy under non-degenerate pumping condition. In: Optics and Lasers in Engineering, 158 .

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Abstract

For the development of microscopic tools that can resolve non-fluorescent samples beyond the diffraction limit, we propose focus-engineered non-degenerate pumped coherent anti-Stokes Raman scattering (CARS) using spatial light modulator (SLM)-based phase shaping, liquid lens focus control, and confocal detection. Non-degenerate pumped CARS (ND-CARS) with frequency-doubled probe pulses resulted in approximately 75% improvement in resolution compared to that of degenerate CARS. Focal adjustment using the liquid lens facilitated the accurate overlapping of three beams. The circular π-phase modulation at the center of the probe-beam wavefront demarcated the net CARS focal volume into a sub 100 nm-scale core and surrounding side lobes. The confocal geometry detection setup successfully removed the side lobes, allowing optical imaging of 81 nm-sized zinc oxide particles at 87 nm, and edge-to-edge resolution was determined to be 103 nm.

Item Type:	Journal Article
Publication:	Optics and Lasers in Engineering
Publisher:	Elsevier Ltd
Additional Information:	The copyright for this article belongs to the Elsevier Ltd.
Keywords:	Coherent anti-Stokes Raman spectroscopy; Nanoparticles; Sub-diffraction-limit nonlinear optical microscopy
Department/Centre:	Division of Electrical Sciences > Electrical Communication Engineering
Date Deposited:	04 Jul 2022 05:16
Last Modified:	04 Jul 2022 05:16
URI:	https://eprints.iisc.ac.in/id/eprint/74112

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