Chromatographic and spectrophotometric analysis of heartwood extracts of four Pterocarpus species

Bisht, SS and Mishra, K and Pandey, KK and Chandra, G and Nayak, MM and Shashikala, S (2022) Chromatographic and spectrophotometric analysis of heartwood extracts of four Pterocarpus species. In: Wood Science and Technology, 56 (2). pp. 459-476.

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Abstract

In this study, chromatographic and spectrophotometric analysis of methanolic extract of heartwood of Pterocarpus santalinus Linn (Red Sanders) collected from trees grown in eight different locations was carried out. Usefulness of these techniques for discrimination of some of the commonly occurring species in Pterocarpus genus was assessed. Thin layer chromatography (TLC) fingerprints of methanolic extract of P. santalinus heartwood revealed distinct spots at retention factor (Rf) 0.94 (light brown), 0.91 (purple), 0.79 (blackish brown), 0.75 (light purple), 0.60 (yellow), 0.51 (dark red) and 0.28 (brownish red) in normal phase. High-performance liquid chromatography (HPLC) analysis indicated at least 11 common peaks at retention time (tR) of 3.1, 3.5, 8.9, 11.1, 12.8, 13.5, 14.9, 15.7, 17.9, 18.3, and 26.5 (minutes). Ultraviolet visible spectrophotometric analysis of methanolic extract of heartwood exhibited a broad absorption spectrum with consistency in major absorbance peaks at ~ 280 nm, ~ 320 nm, ~ 472 nm and ~ 505 nm. Similarities in chromatographic pattern between the P. santalinus samples from different locations were evaluated using principal component analysis. The developed fingerprints of methanolic extract of P. santalinus heartwood were compared with those of Pterocarpus marsupium, Pterocarpus soyauxii, and Pterocarpus macrocarpus. The results demonstrate that HPLC and TLC fingerprints can supplement wood anatomy in accurate identification of above Pterocarpus species. UV visible absorption spectrophotometric analysis can be helpful to differentiate P. santalinus from P. macrocarpus and P. marsupium, but P. santalinus and P. soyauxii could not be distinguished using this technique. © 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

Item Type:	Journal Article
Publication:	Wood Science and Technology
Publisher:	Springer Science and Business Media Deutschland GmbH
Additional Information:	The copyright for this article belongs to the Springer Science and Business Media Deutschland GmbH.
Keywords:	Absorption spectroscopy; Principal component analysis; Spectrophotometers; Spectrophotometry; Thin layer chromatography; Wood products, Absorbance peak; Common peak; High-performance liquid chromatography; Methanolic extracts; Normal phase; Pterocarpus santalinus; Retention factors; Retention time; Spectrophotometric analysis; Thin-layer chromatography, High performance liquid chromatography, Analysis; Pterocarpus Macrocarpus; Reaction Time; Spectrometers; Spectroscopy; Thin Layer Chromatography; Wood Products
Department/Centre:	Division of Interdisciplinary Sciences > Centre for Nano Science and Engineering
Date Deposited:	15 Jun 2022 10:04
Last Modified:	15 Jun 2022 10:04
URI:	https://eprints.iisc.ac.in/id/eprint/73784

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