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A proposed unified mitotic chromosome architecture

Sedat, J and McDonald, A and Kasler, H and Verdin, E and Cang, H and Arigovindan, M and Murre, C and Elbaum, M (2022) A proposed unified mitotic chromosome architecture. In: Proceedings of the National Academy of Sciences of the United States of America, 119 (20).

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Official URL: https://doi.org/10.1073/pnas.2119107119


A molecular architecture is proposed for a representative mitotic chromosome, human chromosome 10. This architecture is built on an interphase chromosome structure based on cryo-electron microscopy (cryo-EM) cellular tomography [J. Sedat et al., Proc. Natl. Acad. Sci. U.S.A., in press], thus unifying chromosome structure throughout the complete mitotic cycle. The basic organizational principle for mitotic chromosomes is specific coiling of the 11-nm nucleosome fiber into large scale, ∼200-nm interphase structures, a Slinky [https://en.wikipedia.org/wiki/Slinky; motif cited in S. Bowerman et al., eLife 10, e65587 (2021)], then further modified with subsequent additional coiling for the final mitotic chromosome structure. The final mitotic chromosome architecture accounts for the dimensional values as well as the well-known cytological configurations. In addition, proof is experimentally provided by digital PCR technology that G1 T cell nuclei are diploid with one DNA molecule per chromosome. Many nucleosome linker DNA sequences, the promotors and enhancers, are suggestive of optimal exposure on the surfaces of the large-scale coils.

Item Type: Journal Article
Publication: Proceedings of the National Academy of Sciences of the United States of America
Publisher: National Academy of Sciences
Additional Information: The copyright for this article belongs to the Authors.
Keywords: article; biophysics; cell nucleus; chromosome structure; controlled study; cryoelectron microscopy; cytology; digital polymerase chain reaction; diploidy; DNA sequence; enhancer region; human; human chromosome; interphase; mitosis; nucleosome; promoter region; structure analysis; T lymphocyte; tomography; United States; genetics; nucleosome, Humans; Interphase; Mitosis; Nucleosomes
Department/Centre: Division of Electrical Sciences > Electrical Engineering
Date Deposited: 24 Jun 2022 10:20
Last Modified: 24 Jun 2022 10:20
URI: https://eprints.iisc.ac.in/id/eprint/73636

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