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A micro-mechanical compliant device for individual cell-stretching, compression, and in-situ force-measurement

Kollimada, Somanna A and Khan, Sambuddha and Balakrishna, Sreenath and Raju, Shilpa and Matad, Suma and Ananthasuresh, GK (2017) A micro-mechanical compliant device for individual cell-stretching, compression, and in-situ force-measurement. In: 1st International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017, 17-21 July 2017, Montreal, QC, Canada, pp. 1-6.

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Official URL: https://doi.org/10.1109/MARSS.2017.8001943

Abstract

We describe the fabrication, characterization and operation of a microfabricated, overhanging micromachined SU-8 device that can stretch and compress individual cells. This compliant device is composed of folded beams with a single actuation point. Upon actuation with a glass micropipette attached to an XYZ positioner, it can apply varying deformation to multiple cells simultaneously. The applied force is computed using the measured displacement of the compliant device. Cell-attachment is achieved by simply adding a suspension of cells on the device. The stiffness of the device was designed such that cells can deform the beams they are attached to. Measuring this deformation in the absence of actuation allows for quantifying the forces exerted by the cells with a resolution of 0.18 μΝ.

Item Type: Conference Paper
Publisher: Institute of Electrical and Electronics Engineers Inc.
Additional Information: The Copyright of this article belongs to the Institute of Electrical and Electronics Engineers Inc.
Keywords: Cell manipulation; Cellular force; Folded beam; Microfabrication; Su-8; Cells; Cytology; Deformation; Microfabrication; Robotics; Cell attachments; Cell manipulation; Cellular force; Compliant devices; Folded-beam; Individual cells; Micro-mechanical; Su-8; Molecular biology
Department/Centre: Division of Mechanical Sciences > Mechanical Engineering
Date Deposited: 13 Jun 2022 04:56
Last Modified: 13 Jun 2022 04:56
URI: https://eprints.iisc.ac.in/id/eprint/73298

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