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Cellular Delivery of siRNAs Using Bolaamphiphiles

Gupta, Kshitij (2017) Cellular Delivery of siRNAs Using Bolaamphiphiles. [Book Chapter]

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Official URL: https://doi.org/10.1007/978-1-4939-7138-1_12


Discovery of RNA interference (RNAi) has opened up a new arena of therapeutic intervention for the treatment of cancerous as well as noncancerous diseases. The RNAi pathway utilizes RNAi inducers such as small interfering RNAs (siRNAs) to target and silence disease causing genes. However, efficient delivery of siRNAs for eliciting efficacious RNAi has remained a daunting challenge. Nonviral vectors such as lipids have shown great promise in delivering siRNAs. Recently, a novel class of cationic lipid molecules “bolaamphiphile lipids” or “bola lipids” has been shown to deliver siRNAs to cause effective gene silencing in cells. The present chapter showcases the ability of bola lipids to form micelles, bind with nucleic acids and protect nucleic acids against nucleases. Also, high in vitro transfection efficiency for silencing green fluorescent protein (GFP) using Dicer substrate siRNAs (dsiRNAs) designed against GFP at nontoxic dose in a human breast cancer model is demonstrated. Our results showed that these cationic bola lipids are promising siRNA delivery agents. Eckart Bindewald, Bruce A. Shapiro

Item Type: Book Chapter
Series.: Methods in Molecular Biology
Publisher: Humana Press Inc.
Additional Information: The Copyright of this article belong to the Humana Press Inc.
Keywords: Bolaamphiphiles; dsiRNAs; Green fluorescent protein; Human breast cancer cells; RNAi; RNA–DNA hybrids; Cell Line, Tumor; Cell Survival; Deoxyribonucleases; DNA; Furans; Gene Expression; Gene Silencing; Gene Transfer Techniques; Genes, Reporter; Humans; Nucleic Acid Conformation; Nucleic Acids; Pyridones; RNA, Small Interfering; Transfection; amphophile; bolaamphiphile; complementary DNA; double stranded DNA; nuclease; nucleic acid; small interfering RNA; unclassified drug; bolaamphiphile; deoxyribonuclease; DNA; furan derivative; nucleic acid; pyridone derivative; small interfering RNA; anisotropy; cell viability; controlled study; cryoelectron microscopy; DNA degradation; DNA RNA hybridization; flow cytometer; fluorescence activated cell sorting; fluorescence polarization; fluorescent anisotropy; gene targeting; human; human cell; in vitro gene transfer; micelle; photon correlation spectroscopy; cell survival; chemistry; conformation; gene expression; gene silencing; gene transfer; genetic transfection; genetics; procedures; reporter gene; tumor cell line
Department/Centre: Division of Chemical Sciences > Inorganic & Physical Chemistry
Date Deposited: 27 May 2022 04:31
Last Modified: 31 May 2022 05:05
URI: https://eprints.iisc.ac.in/id/eprint/72567

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