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Structural impact of thioamide incorporation into a β-hairpin

Fiore, Kristen E and Patist, Martijn J and Giannakoulias, Sam and Huang, Cheng-Hsin and Verma, Hitesh and Khatri, Bhavesh and Cheng, Richard P and Chatterjee, Jayanta and Petersson, E James (2022) Structural impact of thioamide incorporation into a β-hairpin. In: RSC Chemical Biology, 3 (5). pp. 582-591. ISSN 2633-0679

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Official URL: https://doi.org/10.1039/d1cb00229e


The thioamide is a naturally-occurring single atom substitution of the canonical amide bond. The exchange of oxygen to sulfur alters the amide's physical and chemical characteristics, thereby expanding its functionality. Incorporation of thioamides in prevalent secondary structures has demonstrated that they can either have stabilizing, destabilizing, or neutral effects. We performed a systematic investigation of the structural impact of thioamide incorporation in a β-hairpin scaffold with nuclear magnetic resonance (NMR). Thioamides as hydrogen bond donors did not increase the foldedness of the more stable “YKL” variant of this scaffold. In the less stable “HPT” variant of the scaffold, the thioamide could be stabilizing as a hydrogen bond donor and destabilizing as a hydrogen bond acceptor, but the extent of the perturbation depended upon the position of incorporation. To better understand these effects we performed structural modelling of the macrocyclic folded HPT variants. Finally, we compare the thioamide effects that we observe to previous studies of both side-chain and backbone perturbations to this β-hairpin scaffold to provide context for our observations.

Item Type: Journal Article
Publication: RSC Chemical Biology
Publisher: Royal Society of Chemistry
Additional Information: The copyright of this article belongs to the Authors.
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited: 23 May 2022 04:58
Last Modified: 23 May 2022 04:58
URI: https://eprints.iisc.ac.in/id/eprint/72417

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