ePrints@IIScePrints@IISc Home | About | Browse | Latest Additions | Advanced Search | Contact | Help

Digital light processing-based 3D bioprinting of κ-carrageenan hydrogels for engineering cell-loaded tissue scaffolds

Kumari, Sushma and Mondal, Pritiranjan and Chatterjee, Kaushik (2022) Digital light processing-based 3D bioprinting of κ-carrageenan hydrogels for engineering cell-loaded tissue scaffolds. In: Carbohydrate Polymers, 290 . ISSN 01448617

[img] PDF
car_pol_290_2022 .pdf - Published Version
Restricted to Registered users only

Download (11MB) | Request a copy
Official URL: https://doi.org/10.1016/j.carbpol.2022.119508


The demand to regenerate biological tissues and organs in patients as an alternative to transplants has motivated the tissue engineering field. Digital light processing (DLP)-based three-dimensional (3D) bioprinting technology enables the rapid fabrication of complex 3D cell-laden scaffolds for tissue engineering applications. Herein, we demonstrate the outstanding printability of photocurable methacrylate-κ-carrageenan (MA-κ-CA) using DLP 3D printing. 3D printed hydrogels with varying concentrations (1–5% w/v) of MA-κ-CA were thoroughly characterized for their swelling, degradation, mechanical, and rheological properties, and suitability for bioprinting with living cells. Viscosity and shear thinning behavior of MA-κ-CA faithfully recapitulate the biomechanical properties of soft human tissues. Encapsulated NIH-3T3 cells show high viability and good proliferation over several days. Furthermore, highly complex 3D hydrogel scaffolds of MA-κ-CA were printed to recapitulate the biological complexity of tissues and organs. This work presents a polysaccharide bioink for preparing tissue scaffolds by DLP 3D bioprinting.

Item Type: Journal Article
Publication: Carbohydrate Polymers
Publisher: Elsevier Ltd
Additional Information: The copyright of this article belongs to the Elsevier Ltd.
Keywords: 3D bioprinting; Carbohydrates; Digital light processing; Hydrogels; Tissue engineering
Department/Centre: Division of Mechanical Sciences > Materials Engineering (formerly Metallurgy)
Date Deposited: 20 May 2022 10:38
Last Modified: 20 May 2022 10:38
URI: https://eprints.iisc.ac.in/id/eprint/72313

Actions (login required)

View Item View Item