Impact of embryonic expression of enhanced green fluorescent protein on early mouse development

Devgan, Vikram and Rao, Manchanahalli RS and Seshagiri, Polani B (2004) Impact of embryonic expression of enhanced green fluorescent protein on early mouse development. In: Biochemical and Biophysical Research Communications, 313 (4). pp. 1030-1036.

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Abstract

The impact of embryonic enhanced green fluorescent protein (EGFP)-expression on development is not clear. In this study, we comprehensively assessed EGFP-expression pattern and its effect on early mouse development, following pronuclear-microinjection of the EGFP-transgene, containing chicken-$\beta$-actin promoter and cytomegalovirus enhancer. Preimplantation embryos exhibited differential EGFP-expression patterns. While blastocyst development of non-expressing embryos was 77.3$\pm$1.8%, that of expressing embryos was only 43.9$\pm$1.6% (P < 0.0001). Developmental competence of embryos negatively correlated (r = -0.99) with the levels of EGFP-expression. Faint-, moderate-, and intense-expressing embryos developed to 83.1$\pm$5.3%, 50$\pm$5%, and 9.5$\pm$3.9% blastocysts, respectively (P < 0.002). Interestingly, blastocysts expressing faint–moderate levels of EGFP were developmentally competent through the post-implantation period and delivered viable transgenic ‘green’ mice, following embryo transfer. These results indicate that hyper-expression of EGFP affects preimplantation development and faint–moderate level of its expression is compatible with normal embryogenesis in the mouse.

Item Type:	Journal Article
Publication:	Biochemical and Biophysical Research Communications
Publisher:	Elsevier
Additional Information:	The copyright belongs to Elsevier.
Keywords:	Enhanced green fluorescent protein;Preimplantation embryo;Differential expression;Early development (mouse);Transgenesis
Department/Centre:	Division of Biological Sciences > Molecular Reproduction, Development & Genetics Division of Biological Sciences > Biochemistry
Date Deposited:	31 May 2006
Last Modified:	19 Sep 2010 04:28
URI:	http://eprints.iisc.ac.in/id/eprint/7182

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