Ray, Partho Sarothi and Grover, Richa and Das, Saumitra (2006) Two internal ribosome entry sites mediate the translation of p53 isoforms. In: EMBO Reports, 7 (4). pp. 404-410.
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The p53 tumour suppressor protein has a crucial role in cell-cycle arrest and apoptosis. Previous reports show that the p53 messenger RNA is translated to produce an amino-terminal-deleted isoform $({\triangle}N-p53)$ from an internal initiation codon, which acts as a dominant-negative inhibitor of full-length p53. Here, we show that two internal ribosome entry sites (IRESs) mediate the translation of both full-length and ${\triangle}N-p53$ isoforms. The IRES directing the translation of full-length p53 is in the 5'-untranslated region of the mRNA, whereas the IRES mediating the translation of ${\triangle}N-p53$ extends into the protein-coding region. The two IRESs show distinct cell-cycle phase-dependent activity, with the IRES for full-length p53 being active at the G2–M transition and the IRES for ${\triangle}N-p53$ showing highest activity at the G1–S transition. These results indicate a novel translational control of p53 gene expression and activity.
| Item Type: | Journal Article |
|---|---|
| Publication: | EMBO Reports |
| Publisher: | Nature |
| Additional Information: | Copyright of this articles belongs to Nature Publishing Group. |
| Department/Centre: | Division of Biological Sciences > Microbiology & Cell Biology |
| Date Deposited: | 31 May 2006 |
| Last Modified: | 27 Aug 2008 12:05 |
| URI: | http://eprints.iisc.ac.in/id/eprint/7171 |
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