Pulusu, CP and Manivannan, B and Raman, SS and Singh, S and Khamari, B and Lama, M and Peketi, ASK and Datta, C and Prasad, KN and Nagaraja, V and Pradeep, BE (2022) Localized outbreaks of Pseudomonas aeruginosa belonging to international high-risk clones in a south Indian hospital. In: Journal of medical microbiology, 71 (3).
Full text not available from this repository.Abstract
Introduction. Pseudomonas aeruginosa is now considered as a major bacterial pathogen associated with hospital infections. Frequently, multidrug-resistant (MDR) and extensively drug-resistant (XDR) P. aeruginosa are being encountered. Unusual increase in the P. aeruginosa infections led to the suspicion of outbreaks in the urology ward and cardiothoracic and vascular surgery intensive care unit (CTVS-ICU).Hypothesis. We hypothesize that the localized outbreaks may have originated from environmental sources within the hospital premises. An alternative possibility is the transmission from a previously infected patient or hospital attendant. Understanding the drug-resistance profile and genome characteristics of these clinical samples would determine the likely source of infection and spread.Aim. To perform epidemiological and molecular investigations on the suspected outbreaks of P. aeruginosa in the study centre and identify potential sources of infection.Methodology. Fourteen drug-resistant P. aeruginosa isolated from patients of the urology ward, CTVS-ICU and tap waters collected during the suspected outbreaks were subjected to microbiological and genomic analysis. Comparative genome (CG) analysis of these 14 study genomes with 284 complete P. aeruginosa genomes was performed.Results. Multilocus sequence typing analysis revealed that the isolates belonged to five different sequence types (ST235, ST357, ST639, ST654 and ST1203) and clustered into three distinct groups while two CTVS-ICU isolates remained as singletons. Genome analysis distinguished that the outbreaks in the urology ward and CTVS-ICU are independent, epidemiologically unrelated to each other and with the tap-water isolates.Conclusion. This study highlights the presence of distinct, clonally unrelated, drug-resistant P. aeruginosa within a hospital setting. The genome analysis of the two localized outbreaks revealed their distinct genetic background and phylogenetically unrelated origin. Vigilant screening and effective implementation of infection control measures led to the successful containment of potential environmental reservoirs of P. aeruginosa within the premises.
| Item Type: | Journal Article |
|---|---|
| Publication: | Journal of medical microbiology |
| Publisher: | Microbiology Society |
| Additional Information: | The copyright for this article belongs to the authors. |
| Keywords: | cell clone; epidemic; hospital; human; microbiology; Pseudomonas aeruginosa; Pseudomonas infection, Clone Cells; Disease Outbreaks; Hospitals; Humans; Pseudomonas aeruginosa; Pseudomonas Infections |
| Department/Centre: | Division of Biological Sciences > Microbiology & Cell Biology |
| Date Deposited: | 16 May 2022 06:49 |
| Last Modified: | 16 May 2022 06:49 |
| URI: | https://eprints.iisc.ac.in/id/eprint/71684 |
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