The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking

Bhatt, AM and Setty, SRG (2022) The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking. In: Journal of Visualized Experiments, 2022 (180).

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Abstract

Recycling endosomes (REs) are tubular-vesicular organelles generated from early/ sorting endosomes in all cell types. These organelles play a key role in the biogenesis of melanosomes, a lysosome-related organelle produced by melanocytes. REs deliver the melanocyte-specific cargo to premature melanosomes during their formation. Blockage in the generation of REs, observed in several mutants of Hermansky-Pudlak syndrome, results in hypopigmentation of skin, hair, and eye. Therefore, studying the dynamics (refer to number and length) of REs is useful to understand the function of these organelles in normal and disease conditions. In this study, we aim to measure the RE dynamics using a resident SNARE STX13. © 2022 JoVE Journal of Visualized Experiments.

Item Type:	Journal Article
Publication:	Journal of Visualized Experiments
Publisher:	Journal of Visualized Experiments
Additional Information:	The copyright for this article belongs to Journal of Visualized Experiments
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	21 Mar 2022 07:38
Last Modified:	21 Mar 2022 07:38
URI:	http://eprints.iisc.ac.in/id/eprint/71580

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