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Particle uptake driven phagocytosis in macrophages and neutrophils enhances bacterial clearance

Sharma, P and Vijaykumar, A and Raghavan, JV and Rananaware, SR and Alakesh, A and Bodele, J and Rehman, JU and Shukla, S and Wagde, V and Nadig, S and Chakrabarti, S and Visweswariah, SS and Nandi, D and Gopal, B and Jhunjhunwala, S (2022) Particle uptake driven phagocytosis in macrophages and neutrophils enhances bacterial clearance. In: Journal of Controlled Release, 343 . pp. 131-141.

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Official URL: https://doi.org/10.1016/j.jconrel.2022.01.030


Humans are exposed to numerous synthetic foreign particles in the form of drug delivery systems and diagnostic agents. Specialized immune cells (phagocytes) clear these particles by phagocytosing and attempting to degrade them. The process of recognition and internalization of the particles may trigger changes in the function of phagocytes. Some of these changes, especially the ability of a particle-loaded phagocyte to take up and neutralize pathogens, remains poorly studied. Herein, we demonstrate that the uptake of non-stimulatory cargo-free particles enhances the phagocytic ability of monocytes, macrophages and neutrophils. The enhancement in phagocytic ability was independent of particle properties, such as size or the base material constituting the particle. Additionally, we show that the increased phagocytosis was not a result of cellular activation or cellular heterogeneity but was driven by changes in cell membrane fluidity and cellular compliance. A consequence of the enhanced phagocytic activity was that particulate-laden immune cells neutralize Escherichia coli (E. coli) faster in culture. Moreover, when administered in mice as a prophylactic, particulates enable faster clearance of E. coli and Staphylococcus epidermidis. Together, we demonstrate that the process of uptake induces cellular changes that favor additional phagocytic events. This study provides insights into using non-stimulatory cargo-free particles to engineer immune cell functions for applications involving faster clearance of phagocytosable abiotic and biotic material. © 2022 Elsevier B.V.

Item Type: Journal Article
Publication: Journal of Controlled Release
Publisher: Elsevier B.V.
Additional Information: The copyright for this article belongs to Authors
Keywords: Antigen-antibody reactions; Cytology; Drug delivery; Macrophages; Mammals; Particles (particulate matter), Cellulars; Exposed to; Free particles; Immune cells; Immuno modulations; Micro particles; Monocyte; Particle uptakes; Particulates; Phagocyte, Escherichia coli
Department/Centre: Division of Biological Sciences > Biochemistry
Division of Biological Sciences > Molecular Biophysics Unit
Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Division of Interdisciplinary Sciences > Centre for Biosystems Science and Engineering
Date Deposited: 17 Feb 2022 06:39
Last Modified: 17 Feb 2022 06:39
URI: http://eprints.iisc.ac.in/id/eprint/71318

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