Selective pathological and intracellular detection of human serum albumin by photophysical and electrochemical techniques using a FRET-based molecular probe

Saha, P and Moitra, P and Bhattacharjee, U and Bhattacharya, S (2022) Selective pathological and intracellular detection of human serum albumin by photophysical and electrochemical techniques using a FRET-based molecular probe. In: Biosensors and Bioelectronics, 203 .

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Abstract

A simple, easily synthesizable, low-cost, fluorescent turn-on probe is presented herein for the selective and quantitative detection of human serum albumin (HSA) in different biological fluids collected from patients with various clinical manifestations. The sensor can detect HSA level by both photophysical and electrochemical means. The developed probe is also efficient in rapid quantification of HSA level in single living cell, cell lysate and tissue extract with high sensitivity. Both higher (millimolar) and trace (micromolar) amount of serum albumin can be accurately quantified using this probe in vast array of biomedical samples. This chemical sensor is also used as a part of Förster Resonance Energy Transfer (FRET) based system adding further accuracy to the measurement technique. Intracellular concentrations of HSA can be measured as well as imaged using this newly synthesized probe. Electrochemical detection of HSA concentrations can also be achieved with this biosensor using a potentiostat. Thus, this probe offers a unique potential of diagnosing HSA levels directly in various biological samples, using its bimodal (i.e., photophysical and electrochemical) properties which is hitherto unknown till date. © 2022 Elsevier B.V.

Item Type:	Journal Article
Publication:	Biosensors and Bioelectronics
Publisher:	Elsevier Ltd
Additional Information:	The copyright for this article belongs to Elsevier Ltd
Keywords:	Chemical detection; Diagnosis; Energy transfer; Forster resonance energy transfer; Voltage regulators, Biological fluids; Electrochemical techniques; Human serum albumins; Low-costs; Molecular Probes; Photophysical; Quantitative detection; Resonance energy transfer; Selective detection; Simple++, Probes
Department/Centre:	Division of Chemical Sciences > Organic Chemistry
Date Deposited:	17 Feb 2022 06:37
Last Modified:	17 Feb 2022 06:37
URI:	http://eprints.iisc.ac.in/id/eprint/71307

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