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RapidET: a MEMS-based platform for label-free and rapid demarcation of tumors from normal breast biopsy tissues

G K, AV and Gogoi, G and Behera, B and Rila, S and Rangarajan, A and Pandya, HJ (2022) RapidET: a MEMS-based platform for label-free and rapid demarcation of tumors from normal breast biopsy tissues. In: Microsystems and Nanoengineering, 8 (1).

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Official URL: https://doi.org/10.1038/s41378-021-00337-z


The rapid and label-free diagnosis of malignancies in ex vivo breast biopsy tissues has significant utility in pathology laboratories and operating rooms. We report a MEMS-based platform integrated with microchips that performs phenotyping of breast biopsy tissues using electrothermal sensing. The microchip, fabricated on a silicon substrate, incorporates a platinum microheater, interdigitated electrodes (IDEs), and resistance temperature detectors (RTDs) as on-chip sensing elements. The microchips are integrated onto the platform using a slide-fit contact enabling quick replacement for biological measurements. The bulk resistivity (�B), surface resistivity (�S), and thermal conductivity (k) of deparaffinized and formalin-fixed paired tumor and adjacent normal breast biopsy samples from N = 8 patients were measured. For formalin-fixed samples, the mean �B for tumors showed a statistically significant fold change of 4.42 (P = 0.014) when the tissue was heated from 25 °C to 37 °C compared to the adjacent normal tissue, which showed a fold change of 3.47. The mean �S measurements also showed a similar trend. The mean k of the formalin-fixed tumor tissues was 0.309 ± 0.02 W m�1 K�1 compared to a significantly higher k of 0.563 ± 0.028 W m�1 K�1 for the adjacent normal tissues. A similar trend was observed in �B,�S, and k for the deparaffinized tissue samples. An analysis of a combination of �B, �S, and k using Fisher�s combined probability test and linear regression suggests the advantage of using all three parameters simultaneously for distinguishing tumors from adjacent normal tissues with higher statistical significance. © 2022, The Author(s).

Item Type: Journal Article
Publication: Microsystems and Nanoengineering
Publisher: Springer Nature
Additional Information: The copyright for this article belongs to Authors
Keywords: Biopsy; Formaldehyde; Histology; Temperature sensors; Thermal conductivity, Biopsy tissues; Breast biopsies; Ex-vivo; Inter-digitated electrodes; Interdigitated electrodes; Label free; Microheater; Normal tissue; Phenotyping; Silicon substrates, Tumors
Department/Centre: Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Division of Electrical Sciences > Electronic Systems Engineering (Formerly Centre for Electronic Design & Technology)
Division of Interdisciplinary Sciences > Centre for Biosystems Science and Engineering
Division of Mechanical Sciences > Centre for Product Design & Manufacturing
Date Deposited: 08 Feb 2022 10:24
Last Modified: 08 Feb 2022 10:24
URI: http://eprints.iisc.ac.in/id/eprint/71221

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