Chaterji, S and Barik, A and Sathyamurthy, A (2021) Intraspinal injection of adeno-associated viruses into the adult mouse spinal cord. In: STAR Protocols, 2 (3).
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Abstract
Genetic dissection of neural circuits has been accelerated by recent advances in viral-based vectors. This protocol describes an effective approach to performing intraspinal injections of adeno-associated viruses, which can be used to label, manipulate, and monitor spinal and supraspinal neurons. By avoiding invasive laminectomies and restrictive spinal-clamping and by adopting injectable anaesthetics and tough quartz glass micropipettes, our protocol presents a time-saving and efficient approach for genetic manipulation of neural circuits nucleated in the spinal cord. For complete details on the use and execution of this protocol, please refer to Sathyamurthy et al. (2020). © 2021 The Authors
| Item Type: | Journal Article |
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| Publication: | STAR Protocols |
| Publisher: | Cell Press |
| Additional Information: | The copyright for this article belongs to Authors |
| Department/Centre: | Division of Biological Sciences > Centre for Neuroscience |
| Date Deposited: | 01 Feb 2022 12:35 |
| Last Modified: | 01 Feb 2022 12:35 |
| URI: | http://eprints.iisc.ac.in/id/eprint/71197 |
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