Naik, HC and Hari, K and Chandel, D and Mandal, S and Jolly, MK and Gayen, S (2021) Semicoordinated allelic-bursting shape dynamic random monoallelic expression in pregastrulation embryos. In: iScience, 24 (9).
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Abstract
Recently, allele-specific single-cell RNA-seq analysis has demonstrated widespread dynamic random monoallelic expression of autosomal genes (aRME) in different cell types. However, the prevalence of dynamic aRME during pregastrulation remains unknown. Here, we show that dynamic aRME is widespread in different lineages of pregastrulation embryos. Additionally, the origin of dynamic aRME remains elusive. It is believed that independent transcriptional bursting from each allele leads to dynamic aRME. Here, we show that allelic burst is not perfectly independent; instead it happens in a semicoordinated fashion. Importantly, we show that semicoordinated allelic bursting of genes, particularly with low burst frequency, leads to frequent asynchronous allelic bursting, thereby contributing to dynamic aRME. Furthermore, we found that coordination of allelic bursting is lineage specific and genes regulating the development have a higher degree of coordination. Altogether, our study provides significant insights into the prevalence and origin of dynamic aRME and their developmental relevance during early development. © 2021 The Authors
| Item Type: | Journal Article |
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| Publication: | iScience |
| Publisher: | Elsevier Inc. |
| Additional Information: | The copyright for this article belongs to Authors |
| Department/Centre: | Division of Biological Sciences > Molecular Reproduction, Development & Genetics Division of Interdisciplinary Sciences > Centre for Biosystems Science and Engineering |
| Date Deposited: | 01 Feb 2022 12:35 |
| Last Modified: | 01 Feb 2022 12:35 |
| URI: | http://eprints.iisc.ac.in/id/eprint/71196 |
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