Physical-Chemical Characterization of Bilayer Membranes Derived from (±) α-Tocopherol-Based Gemini Lipids and Their Interaction with Phosphatidylcholine Bilayers and Lipoplex Formation with Plasmid DNA

Maiti, B and Kumar, K and Datta, S and Bhattacharya, S (2021) Physical-Chemical Characterization of Bilayer Membranes Derived from (±) α-Tocopherol-Based Gemini Lipids and Their Interaction with Phosphatidylcholine Bilayers and Lipoplex Formation with Plasmid DNA. In: Langmuir .

	PDF lan_2021.pdf - Published Version Restricted to Registered users only Download (8MB) | Request a copy
	PDF la1c01039_si_001.pdf - Published Supplemental Material Restricted to Registered users only Download (1MB) | Request a copy

Abstract

Membrane formation and aggregation properties of two series of (±) α-tocopherol-based cationic gemini lipids without and with hydroxyl functionalities at the headgroup region (TnS n = 3, 4, 5, 6, 8, and 12; THnS n = 4, 5, 6, 8, and 12) with varying polymethylene spacer lengths were investigated extensively while comparing with the corresponding properties of the monomeric counterparts (TM and THM). Liposomal suspensions of each cationic lipid were characterized by dynamic light scattering (DLS), transmission electron microscopy (TEM), zeta potential measurements, and small-angle X-ray diffraction studies. The length of the spacer and the presence of hydroxyl functionalities at the headgroup region strongly contribute to the aggregation behavior of these gemini lipids in water. The interaction of each tocopherol lipid with a model phospholipid, 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC)-derived vesicles, was thoroughly examined by differential scanning calorimetry (DSC) and 1,6-diphenyl-1,3,5-hexatriene (DPH)-doped fluorescence anisotropy measurements. The binding efficiency of the cationic tocopherol liposomes with plasmid DNA (pDNA) was followed by an ethidium bromide (EB) exclusion assay and zeta potential measurements, whereas negatively charged micellar sodium dodecyl sulfate (SDS)-mediated release of the pDNA from various preformed pDNA-liposomal complexes (lipoplex) was studied by an ethidium bromide (EB) reintercalation assay. The structural transformation of pDNA upon complexation with liposome was characterized using circular dichroism (CD) spectroscopic measurements. Gemini lipid-pDNA interactions depend on both the presence of hydroxyl functionalities at the headgroups and the length of the spacer chain between the headgroups. Succinctly, we performed a detailed physical-chemical characterization of the membranes formed from cationic monomeric and gemini lipids bearing tocopherol as their hydrophobic backbone and describe the role of inserting the -OH group at the headgroup of such lipids. © 2021 American Chemical Society.

Item Type:	Journal Article
Publication:	Langmuir
Publisher:	American Chemical Society
Additional Information:	The copyright for this article belongs to American Chemical Society
Keywords:	Agglomeration; Biomolecules; Dichroism; Differential scanning calorimetry; DNA; Dynamic light scattering; High resolution transmission electron microscopy; Phospholipids; RNA; Sodium dodecyl sulfate; Sulfur compounds; Zeta potential, Cationics; Chemical characterization; Ethidium bromide; Head groups; Hydroxyl functionality; Lipoplexes; Monomerics; Plasmid DNA; Zeta potential measurements; α-Tocopherol, Liposomes
Department/Centre:	Division of Chemical Sciences > Organic Chemistry
Date Deposited:	20 Jan 2022 06:55
Last Modified:	20 Jan 2022 06:55
URI:	http://eprints.iisc.ac.in/id/eprint/71000

Actions (login required)

View Item