Ghosh, G and Barman, R and Mukherjee, A and Ghosh, U and Ghosh, S and Fernández, G (2021) Control over Multiple Nano- and Secondary Structures in Peptide Self-Assembly. In: Angewandte Chemie - International Edition .
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Abstract
Herein, we report the rich morphological and conformational versatility of a biologically active peptide (PEP-1), which follows diverse self-assembly pathways to form up to six distinct nanostructures and up to four different secondary structures through subtle modulation in pH, concentration and temperature. PEP-1 forms twisted β-sheet secondary structures and nanofibers at pH 7.4, which transform into fractal-like structures with strong β-sheet conformations at pH 13.0 or short disorganized elliptical aggregates at pH 5.5. Upon dilution at pH 7.4, the nanofibers with twisted β-sheet secondary structural elements convert into nanoparticles with random coil conformations. Interestingly, these two self-assembled states at pH 7.4 and room temperature are kinetically controlled and undergo a further transformation into thermodynamically stable states upon thermal annealing: whereas the twisted β-sheet structures and corresponding nanofibers transform into 2D sheets with well-defined β-sheet domains, the nanoparticles with random coil structures convert into short nanorods with α-helix conformations. Notably, PEP-1 also showed high biocompatibility, low hemolytic activity and marked antibacterial activity, rendering our system a promising candidate for multiple bio-applications. © 2021 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH
| Item Type: | Journal Article |
|---|---|
| Publication: | Angewandte Chemie - International Edition |
| Publisher: | John Wiley and Sons Inc |
| Additional Information: | The copyright for this article belongs to the Author. |
| Keywords: | Biocompatibility; Nanofibers; Nanoparticles; Nanorods; Self assembly, 1-forms; Amphiphilic systems; Biologically active peptides; Ellipticals; Fractallike structures; Nano-structures; Peptide self assemblies; Secondary structural elements; Secondary structures; β-sheet conformations, Peptides |
| Department/Centre: | Division of Chemical Sciences > Organic Chemistry |
| Date Deposited: | 05 Jan 2022 06:39 |
| Last Modified: | 05 Jan 2022 06:39 |
| URI: | http://eprints.iisc.ac.in/id/eprint/70900 |
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