Arabidopsis inositol polyphosphate kinases IPK1 and ITPK1 modulate crosstalk between SA-dependent immunity and phosphate-starvation responses

Gulabani, H and Goswami, K and Walia, Y and Roy, A and Noor, JJ and Ingole, KD and Kasera, M and Laha, D and Giehl, RFH and Schaaf, G and Bhattacharjee, S (2021) Arabidopsis inositol polyphosphate kinases IPK1 and ITPK1 modulate crosstalk between SA-dependent immunity and phosphate-starvation responses. In: Plant Cell Reports .

	PDF pla_cel_rep_2021pdf - Published Version Restricted to Registered users only Download (5MB) | Request a copy
	PDF 299_2021_2812_MOESM1_ESM.pdf - Published Supplemental Material Restricted to Registered users only Download (11MB) | Request a copy

Abstract

Key message: SelectiveArabidopsis thalianainositol phosphate kinase functions modulate response amplitudes in innate immunity by balancing signalling adjustments with phosphate homeostasis networks. Abstract: Pyrophosphorylation of InsP6 generates InsP7 and/or InsP8 containing high-energy phosphoanhydride bonds that are harnessed during energy requirements of a cell. As bona fide co-factors for several phytohormone networks, InsP7/InsP8 modulate key developmental processes. With requirements in transducing jasmonic acid (JA) and phosphate-starvation responses (PSR), InsP8 exemplifies a versatile metabolite for crosstalks between different cellular pathways during diverse stress exposures. Here we show that Arabidopsis thaliana INOSITOL PENTAKISPHOSPHATE 2-KINASE 1 (IPK1), INOSITOL 1,3,4-TRISPHOSPHATE 5/6-KINASE 1 (ITPK1), and DIPHOSPHOINOSITOL PENTAKISPHOSPHATE KINASE 2 (VIH2) implicated in InsP8 biosynthesis, suppress salicylic acid (SA)-dependent immunity. In ipk1, itpk1 or vih2 mutants, constitutive activation of defenses lead to enhanced resistance against the Pseudomonas syringae pv tomato DC3000 (PstDC3000) strain. Our data reveal that upregulated SA-signaling sectors potentiate increased expression of several phosphate-starvation inducible (PSI)-genes, previously known in these mutants. In reciprocation, upregulated PSI-genes moderate expression amplitudes of defense-associated markers. We demonstrate that SA is induced in phosphate-deprived plants, however its defense-promoting functions are likely diverted to PSR-supportive roles. Overall, our investigations reveal selective InsPs as crosstalk mediators in defense-phosphate homeostasis and in reprogramming stress-appropriate response intensities. © 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

Item Type:	Journal Article
Publication:	Plant Cell Reports
Publisher:	Springer Science and Business Media Deutschland GmbH
Additional Information:	The copyright for this article belongs to Springer Science and Business Media Deutschland GmbH
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	13 Dec 2021 11:40
Last Modified:	13 Dec 2021 11:40
URI:	http://eprints.iisc.ac.in/id/eprint/70771

Actions (login required)

View Item