Phagocytic Uptake of Polymeric Particles by Immune Cells under Flow Conditions

Srinivas, M and Sharma, P and Jhunjhunwala, S (2021) Phagocytic Uptake of Polymeric Particles by Immune Cells under Flow Conditions. In: Molecular Pharmaceutics .

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Abstract

Particles injected intravenously are thought to be cleared by macrophages residing in the liver and spleen, but they also encounter circulating immune cells. It remains to be established if the circulating cells can take up particles while flowing and if the uptake capacity is similar under static and flow conditions. Here, we use an in vitro peristaltic pump setup that mimics pulsatile blood flow to determine if immune cells take up particles under constant fluidic flow. We use polystyrene particles of varying sizes as the model of a polymeric particle for these studies. Our results show that the immune cells do phagocytose under flow conditions. We demonstrate that cell lines representing myeloid cells, primary human neutrophils, and monocytes take up submicrometer-sized particles at similar or better rates under flow compared to static conditions. Experiments with whole human blood show that, even under the crowding effects of red blood cells, neutrophils and monocytes take up particles while flowing. Together, these data suggest that circulating immune cells are likely to phagocytose intravenously injected particulates, which has implications for the design of particles to evade or target these cells. ©

Item Type:	Journal Article
Publication:	Molecular Pharmaceutics
Publisher:	American Chemical Society
Additional Information:	The copyright for this article belongs to American Chemical Society
Department/Centre:	Division of Interdisciplinary Sciences > Centre for Biosystems Science and Engineering UG Programme
Date Deposited:	13 Dec 2021 11:39
Last Modified:	13 Dec 2021 11:39
URI:	http://eprints.iisc.ac.in/id/eprint/70767

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