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Single-particle cryo-em reveals conformational variability of the oligomeric vcc β-barrel pore in a lipid bilayer

Sengupta, N and Mondal, AK and Mishra, S and Chattopadhyay, K and Dutta, S (2021) Single-particle cryo-em reveals conformational variability of the oligomeric vcc β-barrel pore in a lipid bilayer. In: Journal of Cell Biology, 220 (12).

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Official URL: https://doi.org/10.1083/JCB.202102035

Abstract

Vibrio cholerae cytolysin (VCC) is a water-soluble, membrane-damaging, pore-forming toxin (PFT) secreted by pathogenic V. cholerae, which causes eukaryotic cell death by altering the plasma membrane permeability. VCC self-assembles on the cell surface and undergoes a dramatic conformational change from prepore to heptameric pore structure. Over the past few years, several high-resolution structures of detergent-solubilized PFTs have been characterized. However, high-resolution structural characterization of small β-PFTs in a lipid environment is still rare. Therefore, we used single-particle cryo-EM to characterize the structure of the VCC oligomer in large unilamellar vesicles, which is the first atomic-resolution cryo-EM structure of VCC. From our study, we were able to provide the first documented visualization of the rim domain amino acid residues of VCC interacting with lipid membrane. Furthermore, cryo-EM characterization of lipid bilayer–embedded VCC suggests interesting conformational variabilities, especially in the transmembrane channel, which could have a potential impact on the pore architecture and assist us in understanding the pore formation mechanism.

Item Type: Journal Article
Publication: Journal of Cell Biology
Publisher: Rockefeller University Press
Additional Information: The copyright for this article belongs to Rockefeller University Press.
Keywords: Bacterial Proteins; Cell Membrane; Cryoelectron Microscopy; Lipid Bilayers; Models, Molecular; Perforin; Protein Multimerization; Protein Structure, Secondary; Vibrio cholerae
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited: 13 Dec 2021 11:38
Last Modified: 06 Jun 2023 10:05
URI: https://eprints.iisc.ac.in/id/eprint/70761

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