α-Helices propagating from stable nucleators exhibit unconventional thermal folding

Pal, S and Banerjee, S and Prabhakaran, EN (2021) α-Helices propagating from stable nucleators exhibit unconventional thermal folding. In: FEBS Letters .

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Abstract

Although the effect of thermal perturbations on protein structure has long been modeled in helical peptides, several details, such as the relation between the thermal stabilities of the propagating and nucleating segments of helices, remain elusive. We had earlier reported on the helix-nucleating propensities of covalent H-bond surrogate-constrained α-turns. Here, we analyze the thermal stabilities of helices that propagate along peptides appended to these α-helix nucleators using their NMR and far-UV CD spectra. Unconventional thermal folding of these helix models reveals that the helical fold in propagating backbones resists thermal perturbations as long as their nucleating template is intact. The threshold temperature of such resistance is also influenced by the extent of similarity between the natures of helical folds in the nucleating and propagating segments. Correlations between helicities and rigidities of helix-nucleating and helix-propagating segments reveal subtle interdependence, which explains cooperativity and residual helix formation during protein folding. © 2021 Federation of European Biochemical Societies

Item Type:	Journal Article
Publication:	FEBS Letters
Publisher:	John Wiley and Sons Inc
Additional Information:	The copyright for this article belongs to John Wiley and Sons Inc
Department/Centre:	Division of Chemical Sciences > Inorganic & Physical Chemistry
Date Deposited:	18 Nov 2021 11:26
Last Modified:	18 Nov 2021 11:26
URI:	http://eprints.iisc.ac.in/id/eprint/70571

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