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Soluble human leukocyte antigen-G is a potential embryo viability biomarker and a positive predictor of live-births in humans

Vani, V and Vasan, SS and Adiga, SK and Varsha, SR and Sachdeva, G and Kumar, P and Seshagiri, PB (2021) Soluble human leukocyte antigen-G is a potential embryo viability biomarker and a positive predictor of live-births in humans. In: American Journal of Reproductive Immunology .

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Official URL: https://doi.org/10.1111/aji.13499


Problem: Human infertility affects 15�20 of reproductive-age couples and it is mitigated by assisted reproductive technology (ART) approaches. Poor biological viability of embryos contributes to implantation failure and live birth rate (LBR). This study is aimed to examine whether or not embryo-secreted soluble human leukocyte antigen-G (sHLA-G) is (i) associated with developing embryos and (ii) able to predict successful pregnancy outcome. Method of study: A retrospective, multicentric study using 539 human embryo spent medium samples (E-SMs), analysed for sHLA-G levels by ELISA. Correlation analysis was performed on sHLA-G levels with developing embryonic stages, their quality scores and pregnancy outcome in terms of LBR. Results: Of 539 E-SMs analysed, 445 had detectable sHLA-G (83) with levels varying within and across clinics and, between stages of embryonic development. Levels of sHLA-G (ng/mL) were significantly (P <.05) different in E-SMs of cleavage-stage embryos versus blastocysts. There was an insignificant correlation between the sHLA-G levels and morphology scores of embryos. But, sHLA-G levels showed a positive correlation with grades of blastocysts and importantly, its levels were significantly (P <.05) higher in live-birth vis-a-vis no-birth cases. Also, levels were higher in live-births out of blastocysts-ETs versus cleavage-stage-embryo transfers. Altered levels were observed with embryos, which resulted in miscarriages. Overall, a significant (P <.0001) association of sHLA-G with live births was observed. Conclusion: Embryo-derived sHLA-G can be a valuable embryo viability, independent, biomarker, which can predict live-birth outcome and it could be useful as an adjunct to existing criteria for elective single embryo transfer. © 2021 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Item Type: Journal Article
Publication: American Journal of Reproductive Immunology
Publisher: John Wiley and Sons Inc
Additional Information: The copyright for this article belongs to John Wiley and Sons Inc
Department/Centre: Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Date Deposited: 22 Nov 2021 09:32
Last Modified: 22 Nov 2021 09:32
URI: http://eprints.iisc.ac.in/id/eprint/70569

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