Multicentric evaluation of a novel point of care electrochemical ELISA platform for SARS-CoV-2 specific IgG and IgM antibody assay

Kumar, V and Ghosh, K and Chandran, A and Panwar, S and Bhat, A and Konaje, S and Das, S and Srikanta, S and Jaganathan, L and Prasad, S and Venkatesh, DB and Shivaram, C and Swamy, PRK and Bhat, N (2021) Multicentric evaluation of a novel point of care electrochemical ELISA platform for SARS-CoV-2 specific IgG and IgM antibody assay. In: Journal of Virological Methods, 298 .

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Abstract

New diagnostics technologies for the efficient detection and quantification of SARS-CoV-2 antibodies are very crucial to manage the COVID-19 pandemic, especially in the context of emerging vaccination paradigms. Herein, we report on a novel point-of-care Electrochemical ELISA platform with disposable screen printed electrodes functionalized with SARS-CoV-2 Spike Glycoprotein S1, to enable fast and accurate quantitative estimation of total antibody concentration (IgG and IgM) in clinical samples. The quantification is performed with a comparison of electrochemical redox current against the current produced by the spiked monoclonal antibodies with known concentration. The assay is validated through multicentric evaluation against 3 different FDA authorized Laboratory standard techniques, using both EDTA whole blood and serum samples. We demonstrate that the proposed assay has excellent sensitivity and specificity, making it a suitable candidate for epidemiological surveys and quantification of antibodies in COVID-19 vaccination programs. © 2021

Item Type:	Journal Article
Publication:	Journal of Virological Methods
Publisher:	Elsevier B.V.
Additional Information:	The copyright for this article belongs to Author
Department/Centre:	Division of Interdisciplinary Sciences > Centre for Nano Science and Engineering
Date Deposited:	28 Nov 2021 09:52
Last Modified:	28 Nov 2021 09:52
URI:	http://eprints.iisc.ac.in/id/eprint/70329

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