Waturuocha, UW and Athira, PJ and Singh, KK and Malhotra, V and Krishna, MS and Saini, DK (2021) A high-frequency single nucleotide polymorphism in the MtrB sensor kinase in clinical strains of Mycobacterium tuberculosis alters its biochemical and physiological properties. In: PLoS ONE, 16 (9 Sept).
|
PDF
plo_one_16-09_2021.pdf - Published Version Download (2MB) | Preview |
Abstract
The DNA polymorphisms found in clinical strains of Mycobacterium tuberculosis drive altered physiology, virulence, and pathogenesis in them. Although the lineages of these clinical strains can be traced back to common ancestor/s, there exists a plethora of difference between them, compared to those that have evolved in the laboratory. We identify a mutation present in �80 of clinical strains, which maps in the HATPase domain of the sensor kinase MtrB and alters kinase and phosphatase activities, and affects its physiological role. The changes conferred by the mutation were probed by in-vitro biochemical assays which revealed changes in signaling properties of the sensor kinase. These changes also affect bacterial cell division rates, size and membrane properties. The study highlights the impact of DNA polymorphisms on the pathophysiology of clinical strains and provides insights into underlying mechanisms that drive signal transduction in pathogenic bacteria. © 2021 Waturuocha et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
| Item Type: | Journal Article |
|---|---|
| Publication: | PLoS ONE |
| Publisher: | Public Library of Science |
| Additional Information: | The copyright for this article belongs to Authors |
| Department/Centre: | Division of Biological Sciences > Molecular Reproduction, Development & Genetics Division of Interdisciplinary Sciences > Centre for Biosystems Science and Engineering |
| Date Deposited: | 03 Dec 2021 08:39 |
| Last Modified: | 03 Dec 2021 08:39 |
| URI: | http://eprints.iisc.ac.in/id/eprint/70255 |
Actions (login required)
 |
View Item |
