Rapid diagnosis of Leishmania infection with a portable loop-mediated isothermal amplification device

Puri, M and Brar, HK and Mittal, N and Madan, E and Srinivasan, R and Rawat, K and Moulik, S and Chatterjee, M and Gorthi, SS and Muthuswami, R and Madhubala, R (2021) Rapid diagnosis of Leishmania infection with a portable loop-mediated isothermal amplification device. In: Journal of Biosciences, 46 (4).

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Abstract

L. donovani is an intracellular protozoan parasite, that causes visceral leishmaniasis (VL), and consequently, post-kala azar dermal leishmaniasis (PKDL). Diagnosis and treatment of leishmaniasis is crucial for decreasing its transmission. Various diagnostic techniques like microscopy, enzyme-linked immunosorbent assays (ELISA) and PCR-based methods are used to detect leishmaniasis infection. More recently, loop-mediated isothermal amplification (LAMP) assay has emerged as an ideal diagnostic measure for leishmaniasis, primarily due to its accuracy, speed and simplicity. However, point-of-care diagnosis is still not been tested with the LAMP assay. We have developed a portable LAMP device for the monitoring of Leishmania infection. The LAMP assay performed using our device can detect and amplify as little as 100 femtograms of L. donovani DNA. In a preliminary study, we have shown that the device can also amplify L. donovani DNA present in VL and PKDL patient samples with high sensitivity (100), specificity (98) and accuracy (99), and can be used both for diagnostic and prognostic analysis. To our knowledge, this is the first report to describe the development and application of a portable LAMP device which has the potential to evolve as a point-of-care diagnostic and prognostic tool for Leishmania infections in future. © 2021, Indian Academy of Sciences.

Item Type:	Journal Article
Publication:	Journal of Biosciences
Publisher:	Springer
Additional Information:	The copyright for this article belongs to Authors
Keywords:	Leishmania donovani; Protozoa
Department/Centre:	Division of Physical & Mathematical Sciences > Instrumentation Appiled Physics
Date Deposited:	03 Dec 2021 08:36
Last Modified:	03 Dec 2021 08:36
URI:	http://eprints.iisc.ac.in/id/eprint/70237

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