Optimal sugar release from macroalgal feedstock with dilute acid pretreatment and enzymatic hydrolysis

Hebbale, D and Ramachandra, TV (2021) Optimal sugar release from macroalgal feedstock with dilute acid pretreatment and enzymatic hydrolysis. In: Biomass Conversion and Biorefinery .

PDF bio_con_bio_2021.pdf - Published Version Restricted to Registered users only Download (5MB) | Request a copy

Abstract

Production of bioethanol from macroalgal feedstock involves pretreatment and hydrolysis of biomolecules for release of sugar followed by the fermentation of the sugar. The most commonly used pretreatment method for macroalgal biomass is dilute acid hydrolysis using mineral acids H2SO4 and HCl. The optimization of dilute acid hydrolysis of Ulva lactuca yielded 214.67 mg g�1 using 0.5 N H2SO4, 5 w/w substrate concentration at 121 °C for 45 min, and hydrolysis of Enteromorpha intestinalis yielded 239.94 mg g�1 of reducing sugar using 0.7 N H2SO4, 5 w/w substrate concentration at 120 °C for 45 min. Crude enzyme extracted from marine bacteria Vibrio parahaemolyticus and purified by the two-step purification produced 61.82 yield with 2.97-fold purification. Enzymatic hydrolysis of pretreated macroalgal biomass produced onefold higher reducing sugar than acid hydrolysis for Ulva lactuca (261.76 ± 0.9 mg g�1) and Enteromorpha intestinalis (289.89 ± 2.4 mg g�1). © 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

Item Type:	Journal Article
Publication:	Biomass Conversion and Biorefinery
Publisher:	Springer Science and Business Media Deutschland GmbH
Additional Information:	The copyright for this article belongs to Springer Science and Business Media Deutschland GmbH
Keywords:	Bacteria; Chlorine compounds; Feedstocks; Purification; Sugar industry; Sulfuric acid, Acid hydrolysis; Dilute acid pretreatment; Dilute-acid hydrolysis; Marine bacterium; Pretreatment methods; Reducing sugars; Substrate concentrations; Vibrio parahaemolyticus, Enzymatic hydrolysis
Department/Centre:	Division of Biological Sciences > Centre for Ecological Sciences Division of Mechanical Sciences > Centre for Sustainable Technologies (formerly ASTRA)
Date Deposited:	21 Nov 2021 16:26
Last Modified:	21 Nov 2021 16:26
URI:	http://eprints.iisc.ac.in/id/eprint/69918

Actions (login required)

View Item