Jaladeep, A and Varghese, CN and Sekhar, A (2021) Measuring radiofrequency fields in NMR spectroscopy using offset-dependent nutation profiles. In: Journal of Magnetic Resonance, 330 .
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Abstract
The application of NMR spectroscopy for studying molecular and reaction dynamics relies crucially on the measurement of the magnitude of radiofrequency (RF) fields that are used to nutate or lock the nuclear magnetization. Here, we report a method for measuring RF field amplitudes that leverages the intrinsic modulations observed in offset-dependent NMR nutation profiles of small molecules. Such nutation profiles are exquisitely sensitive to the magnitude of the RF field, and B1 values ranging from 1 to 2000 Hz, as well the inhomogeneity in B1 distributions, can be determined with high accuracy and precision using this approach. In order to measure B1 fields associated with NMR experiments carried out on protein or nucleic acids, where these modulations are obscured by the large transverse relaxation rate constants of the analyte, our approach can be used in conjunction with a suitable external small molecule standard, expanding the scope of the method for large biomolecules. © 2021 Elsevier Inc.
| Item Type: | Journal Article |
|---|---|
| Publication: | Journal of Magnetic Resonance |
| Publisher: | Academic Press Inc. |
| Additional Information: | The copyright for this article belongs to Authors |
| Keywords: | Modulation; Nucleic acids; Rate constants, Inhomogeneities; Large biomolecules; Nuclear magnetization; Radio frequencies; Radio-frequency fields; Reaction dynamics; Small molecules; Transverse relaxation rate, Nuclear magnetic resonance spectroscopy |
| Department/Centre: | Division of Biological Sciences > Molecular Biophysics Unit |
| Date Deposited: | 21 Sep 2021 09:34 |
| Last Modified: | 21 Sep 2021 09:34 |
| URI: | http://eprints.iisc.ac.in/id/eprint/69785 |
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