A rapid aptamer-based fluorescence assay for the detection of lipopolysaccharides using SYBR Green I

Radhika, NK and Kamali R, V and Gorthi, SS (2021) A rapid aptamer-based fluorescence assay for the detection of lipopolysaccharides using SYBR Green I. In: Luminescence .

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Abstract

Lipopolysaccharides (endotoxins), found on Gram-negative bacteria, can trigger a severe immune response in humans leading to septic shock and in extreme cases, even death. Therefore, the detection and neutralization of lipopolysaccharides (LPS) is of utmost importance in the pharmaceutical and medical industries. The United States Food and Drug Administration (US FDA) recommended detection method for LPS, the Limulus amebocyte lysate (LAL) assay, is expensive, time consuming, complex, and is prone to interference from proteases. As an alternative, this paper proposes a rapid, label-free fluorescence-based assay using LPS-specific aptamers and the SYBR Green DNA stain. The proposed method has a detection limit of 0.1 ng/ml, which is sufficient to detect the permissible levels of LPS in many pharmaceutical drugs and medical products. The fluorescence signal was found to be a linear function of the concentration of LPS in the range from 0.1 ng/ml to 105 ng/ml. © 2021 John Wiley & Sons Ltd.

Item Type:	Journal Article
Publication:	Luminescence
Publisher:	John Wiley and Sons Ltd
Additional Information:	The copyright for this article belongs to John Wiley and Sons Ltd
Department/Centre:	Division of Physical & Mathematical Sciences > Instrumentation Appiled Physics
Date Deposited:	02 Sep 2021 08:59
Last Modified:	02 Sep 2021 08:59
URI:	http://eprints.iisc.ac.in/id/eprint/69637

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