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Analysis of the Role of Oligosaccharides in the Apoptotic Activity of Glycodelin A

Jayachandran, Rajesh and Shaila, MS and Karande, Anjali A (2004) Analysis of the Role of Oligosaccharides in the Apoptotic Activity of Glycodelin A. In: Journal of Biological Chemistry, 279 (10). pp. 8585-8591.

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Glycodelin A, also known as placental protein-14, is a multifunctional glycosylated protein secreted by the uterine endometrium during the early phases of pregnancy.It is a known suppressor of T cell proliferation, inducer of T cell apoptosis, and inhibitor of sperm zona binding. Unlike in contraceptive activity, where the glycans on the molecule have been shown to play a crucial role, mutagenesis of the asparagines at sites of N-linked glycosylation $(Asn^{28}$ and $Asn^{63})$ to glutamine shows that the apoptogenic activity of glycodelin A is executed by the protein backbone. Glycosylation at $Asn^{28}$ appears to play a role in the extracellular secretion of the molecule, as mutation of $Asn^{28}$ resulted in a significant decrease in the amount of secreted protein, and loss of both glycosylation sites reduced the secretion drastically. Our results also suggest that the loss of glycosylation does not affect the dimerization status of the molecule.

Item Type: Journal Article
Publication: Journal of Biological Chemistry
Publisher: American Society for Biochemistry and Molecular Biology
Additional Information: The copyright belongs to American Society for Biochemistry and Molecular Biology.
Department/Centre: Division of Biological Sciences > Biochemistry
Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 27 May 2006
Last Modified: 19 Sep 2010 04:27
URI: http://eprints.iisc.ac.in/id/eprint/6942

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