ePrints@IIScePrints@IISc Home | About | Browse | Latest Additions | Advanced Search | Contact | Help

Application of Raman fiber lasers in a wavelength-dependent cell-viability study for cancerous and healthy cells

Aparanji, S and Kamat, S and Rajashekhar, HM and Supradeepa, VR (2021) Application of Raman fiber lasers in a wavelength-dependent cell-viability study for cancerous and healthy cells. In: Optical Interactions with Tissue and Cells XXXII 2021, 6-11 Mar 2021, virtual, online.

[img] PDF
SPIE_2021_Santosh A.pdf - Published Version
Restricted to Registered users only

Download (553kB)
Official URL: https://doi.org/10.1117/12.2583084


The fields of photodynamic therapy (PDT) and radiation therapy customarily rely on lasers operating at a fixed wavelength (typically 1064 nm Nd:YAG laser), primarily because of the traditional availability of such lasers. However, Raman fiber lasers have made concurrent technological progress to emerge as wavelength-agile laser sources, capable of providing high laser powers at any wavelength, primarily from the 1 -2.0 um wavelengths. In this work, we explore for the first time, the use of a high power, wavelength-tunable Raman fiber laser for performing a wavelength-dependent cell-killing effect study on cancerous and healthy cell lines. Specifically, we irradiate at different wavelengths (from 1 um to 1.6 um) breast cancer cells and healthy cells from a cell line, cultured in well plates. Our in-house built Raman laser is power-tunable apart from being wavelength-tunable, the power and duration of irradiation was optimised for achieving the best contrast between viability of cancerous vs. healthy cells. Flow cytometry is used for cell-viability tests. The results give interesting insights on the choice of wavelengths and we show that 1064 nm lasers traditionally used are not the best choice of wavelength to use for this application while 1480nm lasers performed best. We conclusively demonstrate that other wavelengths exist for achieving the best death rate in cancerous cells, leaving healthy cells unharmed. This can pave the way for deployment of Raman fiber lasers as an alternative laser source for this application which can tune the output wavelength to optimize the required laser tissue interaction. For the keywords, select up to 8 key terms for a search on your manuscript's subject. © 2021 SPIE.

Item Type: Conference Paper
Publication: Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Publisher: SPIE
Additional Information: The copyright for this article belongs to SPIE
Keywords: Cell culture; Cells; Fiber lasers; Fibers; Laser tissue interaction; Photodynamic therapy; Radiotherapy; Tissue; Yttrium aluminum garnet, Breast cancer cells; Fixed wavelength; Output wavelengths; Photodynamic therapy (PDT); Raman fiber lasers; Technological progress; Wavelength tunable; Wavelength-agile lasers, Neodymium lasers
Department/Centre: Division of Biological Sciences > Biochemistry
Division of Interdisciplinary Sciences > Centre for Nano Science and Engineering
Date Deposited: 26 Aug 2021 05:35
Last Modified: 26 Aug 2021 10:08
URI: http://eprints.iisc.ac.in/id/eprint/69360

Actions (login required)

View Item View Item