Monitoring microbial growth on a microfluidic lab-on-chip with electrochemical impedance spectroscopic technique

Shaik, S and Saminathan, A and Sharma, D and Krishnaswamy, JA and Mahapatra, DR (2021) Monitoring microbial growth on a microfluidic lab-on-chip with electrochemical impedance spectroscopic technique. In: Biomedical Microdevices, 23 (2).

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Abstract

A continuous rise in the wastes from industrial effluents, bio-waste, and pharmaceuticals has deteriorated surface water and drinking water sources. Standard laboratory tests of total coliform are time-consuming and logistically inefficient for field data generation. Better and portable sensing technologies are needed. This paper reports an electrical impedance spectroscopic technique incorporated in a micro-fluidic chip with interdigitated microelectrodes to monitor the growth of microbial cells. Lag, log, and stationary phases of Escherichia coli cell growth with an integrated electrode are successfully detected, for samples of reverse osmosis water, standard treated tap water, and recycled water respectively. The results indicate that reverse osmosis water has a higher probability of contamination with bacterial pathogens compared to the other two types of water samples when subjected to the same amount of added nutrients. The statistical analysis shows a possible single detection range with higher-order regression, and repeat use of a single chip with the electrode was found to be within an acceptable limit. The interdigitated electrodes exposed to in-situ cell growth conditions and repeated electrical measurements have shown a promise for possible periodic or continuous monitoring. The paper further identifies several complimentary analysis methodologies that are robust towards phase noise in the measured impedance and are suited particularly for early-stage detection of bacterial contamination. The cell adhesion tendencies over the microelectrode due to the electric field need to be further analyzed. © 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.

Item Type:	Journal Article
Publication:	Biomedical Microdevices
Publisher:	Springer
Additional Information:	The copyright for this article belongs to Springer
Keywords:	Cell adhesion; Cell growth; Cell proliferation; Electric fields; Escherichia coli; Growth kinetics; Microelectrodes; Microfluidics; Phase noise; Potable water; Reverse osmosis; Sewage; Surface waters; Water treatment, Bacterial contamination; Drinking water sources; Electrical measurement; Electrochemical impedance; Escherichia coli cells; Inter-digitated electrodes; Interdigitated microelectrodes; Spectroscopic technique, Effluents
Department/Centre:	Division of Mechanical Sciences > Aerospace Engineering(Formerly Aeronautical Engineering)
Date Deposited:	11 Aug 2021 10:53
Last Modified:	11 Aug 2021 10:53
URI:	http://eprints.iisc.ac.in/id/eprint/69091

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