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Lepidopteran wing scales contain abundant cross-linked film-forming histidine-rich cuticular proteins

Liu, J and Chen, Z and Xiao, Y and Asano, T and Li, S and Peng, L and Chen, E and Zhang, J and Li, W and Zhang, Y and Tong, X and Kadono-Okuda, K and Zhao, P and He, N and Arunkumar, KP and Gopinathan, KP and Xia, Q and Willis, JH and Goldsmith, MR and Mita, K (2021) Lepidopteran wing scales contain abundant cross-linked film-forming histidine-rich cuticular proteins. In: Communications Biology, 4 (1).

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Official URL: https://doi.org/10.1038/s42003-021-01996-4

Abstract

Scales are symbolic characteristic of Lepidoptera; however, nothing is known about the contribution of cuticular proteins (CPs) to the complex patterning of lepidopteran scales. This is because scales are resistant to solubilization, thus hindering molecular studies. Here we succeeded in dissolving developing wing scales from Bombyx mori, allowing analysis of their protein composition. We identified a distinctive class of histidine rich (His-rich) CPs (6�45) from developing lepidopteran scales by LC-MS/MS. Functional studies using RNAi revealed CPs with different histidine content play distinct and critical roles in constructing the microstructure of the scale surface. Moreover, we successfully synthesized films in vitro by crosslinking a 45 His-rich CP (BmorCPR152) with laccase2 using N-acetyl- dopamine or N-β-alanyl-dopamine as the substrate. This molecular study of scales provides fundamental information about how such a fine microstructure is constructed and insights into the potential application of CPs as new biomaterials. © 2021, The Author(s).

Item Type: Journal Article
Publication: Communications Biology
Publisher: Nature Research
Additional Information: The copyright for this article belongs to Authors
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 11 Aug 2021 10:20
Last Modified: 11 Aug 2021 10:20
URI: http://eprints.iisc.ac.in/id/eprint/69085

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