Cancer Stem Cell-Targeted Gene Delivery Mediated by Aptamer-Decorated pH-Sensitive Nanoliposomes

Moitra, P and Misra, SK and Kumar, K and Kondaiah, P and Tran, P and Duan, W and Bhattacharya, S (2021) Cancer Stem Cell-Targeted Gene Delivery Mediated by Aptamer-Decorated pH-Sensitive Nanoliposomes. In: ACS Biomaterials Science and Engineering, 7 (6). pp. 2508-2519.

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Abstract

A new pH-responsive cationic co-liposomal formulation was prepared in this study using the twin version of the amphiphile palmitoyl homocysteine, TPHC; natural zwitterionic lipid, DOPE; and cholesterol-based twin cationic lipid, C5C, at specified molar ratios. This co-liposome was further decorated with a newly designed fluorescently tagged, cholesterol-tethered EpCAM-targeting RNA aptamer for targeted gene delivery. This aptamer-guided nanoliposomal formulation, C5C/DOPE/TPHC at 8:24:1 molar ratio, could efficiently transport the genes in response to low pH of cellular endosomes selectively to the EpCAM overexpressing cancer stem cells. This particular observation was extended using siRNA against GFP to validate their transfection capabilities in response to EpCAM expression. Overall, the aptamer-guided nanoliposomal formulation was found to be an excellent transfectant for in vitro siRNA gene delivery. ©

Item Type:	Journal Article
Publication:	ACS Biomaterials Science and Engineering
Publisher:	American Chemical Society
Additional Information:	The copyright for this article belongs to American Chemical Society
Keywords:	Cholesterol; Diseases; Gene transfer; Genes; Molar ratio, Cancer stem cells; Cationic lipids; Gene Delivery; Homocysteines; Liposomal formulation; PH-responsive; Targeted gene delivery; Zwitterionic lipids, Stem cells
Department/Centre:	Division of Biological Sciences > Molecular Reproduction, Development & Genetics Division of Chemical Sciences > Organic Chemistry
Date Deposited:	19 Jul 2021 10:50
Last Modified:	19 Jul 2021 10:50
URI:	http://eprints.iisc.ac.in/id/eprint/68894

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