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Quantitative Assessment of Labeling Probes for Super-Resolution Microscopy Using Designer DNA Nanostructures

Ganji, M and Schlichthaerle, T and Eklund, AS and Strauss, S and Jungmann, R (2021) Quantitative Assessment of Labeling Probes for Super-Resolution Microscopy Using Designer DNA Nanostructures. In: ChemPhysChem, 22 (10). pp. 911-914.

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Official URL: https://doi.org/10.1002/cphc.202100185


Improving labeling probes for state-of-the-art super-resolution microscopy is becoming of major importance. However, there is currently a lack of tools to quantitatively evaluate probe performance regarding efficiency, precision, and achievable resolution in an unbiased yet modular fashion. Herein, we introduce designer DNA origami structures combined with DNA-PAINT to overcome this issue and evaluate labeling efficiency, precision, and quantification using antibodies and nanobodies as exemplary labeling probes. Whereas current assessment of binders is mostly qualitative, e. g. based on an expected staining pattern, we herein present a quantitative analysis platform of the antigen labeling efficiency and achievable resolution, allowing researchers to choose the best performing binder. The platform can furthermore be readily adapted for discovery and precise quantification of a large variety of additional labeling probes. © 2021 The Authors. ChemPhysChem published by Wiley-VCH GmbH

Item Type: Journal Article
Publication: ChemPhysChem
Publisher: John Wiley and Sons Inc
Additional Information: The copyright for this article belongs to Authors
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 13 Jul 2021 08:58
Last Modified: 13 Jul 2021 08:58
URI: http://eprints.iisc.ac.in/id/eprint/68816

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