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Quantifying molecular aggregation by super resolution microscopy within an excitatory synapse from mouse hippocampal neurons

Kedia, S and Ramanan, N and Nair, D (2021) Quantifying molecular aggregation by super resolution microscopy within an excitatory synapse from mouse hippocampal neurons. In: STAR Protocols, 2 (2).

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Official URL: https://doi.org/10.1016/j.xpro.2021.100470

Abstract

Super-resolution microscopy (SRM) has been widely adopted to probe molecular distribution at excitatory synapses. We present an SRM paradigm to evaluate the nanoscale organization heterogeneity between neuronal subcompartments. Using mouse hippocampal neurons, we describe the identification of the morphological characteristics of nanodomains within functional zones of a single excitatory synapse. This information can be used to correlate structure and function at molecular resolution in single synapses. The protocol can be applied to immunocytochemical/histochemical samples across different imaging paradigms. For complete details on the use and execution of this protocol, please refer to Kedia et al. (2021). © 2021 The Author(s)

Item Type: Journal Article
Publication: STAR Protocols
Publisher: Cell Press
Additional Information: The copyright for this article belongs to Authors
Department/Centre: Division of Biological Sciences > Centre for Neuroscience
Date Deposited: 14 Jul 2021 11:48
Last Modified: 14 Jul 2021 11:48
URI: http://eprints.iisc.ac.in/id/eprint/68779

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