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A dual-channel colorimetric and ratiometric fluorescence chemosensor for detection of Hg2+ ion and its bioimaging applications

Ravichandiran, P and Kaliannagounder, VK and Maroli, N and Boguszewska-Czubara, A and Mas�yk, M and Kim, AR and Park, B-H and Han, M-K and Kim, CS and Park, CH and Yoo, DJ (2021) A dual-channel colorimetric and ratiometric fluorescence chemosensor for detection of Hg2+ ion and its bioimaging applications. In: Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, 257 .

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Official URL: https://doi.org/10.1016/j.saa.2021.119776


A new colorimetric and ratiometric fluorescence chemosensor 4-((3-(octadecylthio)-1,4-dioxo-1,4-dihydronaphthalen-2-yl)amino)benzenesulfonamide (4DBS) was synthesized and investigated for the selective detection of Hg2+ in DMSO-H2O (9:1, v/v) solution. The chemosensor was efficiently synthesized in two steps via Michael-like addition and nucleophilic substitution reactions. The ratiometric fluorescence turn-on response was obtained towards Hg2+, and its fluorescence emission peak was red-shifted by 140 nm with an associated color change from light maroon to pale yellow due to the intramolecular charge transfer effect. The formed coordination metal complex was further evaluated by FT-IR, 1H NMR, and quantum chemical analyses to confirm the binding mechanism. The detection process was sensitive/reversible, and the calculated limit of detection for Hg2+ was 0.451 µM. Furthermore, 4DBS was effectively utilized as a bioimaging agent for detection of Hg2+ in live cells and zebrafish larvae. Additionally, 4DBS showed distinguishing detection of Hg2+ in cancer cells in comparison with normal cells. Thus, 4DBS could be employed as an efficient bioimaging probe for discriminative identification of human cancer cells. © 2021 Elsevier B.V.

Item Type: Journal Article
Publication: Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Publisher: Elsevier B.V.
Additional Information: The copyright for this article belongs to Elsevier B.V.
Keywords: Cells; Charge transfer; Colorimetry; Coordination reactions; Cytology; Diseases; Error detection; Fluorescence; Metal complexes; Metal ions; Metals; Probes; Red Shift; Substitution reactions, Bio-imaging; Cancer cell detection; Chemosensor; Hg2+; Live cell; Metal ion fluorescence; Naphthoquinone probe; Ratiometric fluorescence; Synthesised; Zebrafish imaging, Ketones
Department/Centre: Division of Physical & Mathematical Sciences > Physics
Date Deposited: 15 Jul 2021 10:54
Last Modified: 15 Jul 2021 10:54
URI: http://eprints.iisc.ac.in/id/eprint/68772

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