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Control of Lysogeny in Mycobacteria

Gopinathan, KP and Saroja, D and Gadagkar, R and Ramakrishnan, T (1978) Control of Lysogeny in Mycobacteria. [Book Chapter]

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The Transducing mycobacteriophage l3 is a temperate phage whkh forms turbid plaques of diameter 2- 3 mm on the host Mycobacterium smegmatis SN2. Mutants of the phage have been isolated which form dear plaques. The complementation analysis using different c mutants have shown that there are at least 3 loci oo the phage chromosome involved in lysogenizacion. In addition, certai11 host genes also play a critical role in the establishment of lysogeny by 13. Isolation and characterization of the host mutants in which the phage cannot establish lysogeny, have revealed that the cyclic AMP levels of the host determine che lyric or lysogenic response in mycobaeteria. One-step growth curve experiments indicate chat one cycle of the phage takes 4-5 hours and chat the phage liberation is much more asynchronous in wild type phage infection as compared to c mutant infection.

Item Type: Book Chapter
Publisher: Gustav Fischer Verlag
Additional Information: copyright to this article belongs to Gustav Fischer Verlag - Stuttgart - New York
Keywords: Lysogeny Control, Mycobacteria, Genetics, Experiment, Mutants, Bacteria
Department/Centre: Division of Biological Sciences > Centre for Ecological Sciences
Date Deposited: 17 Mar 2021 10:33
Last Modified: 17 Mar 2021 10:33
URI: http://eprints.iisc.ac.in/id/eprint/68345

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