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Etched Fiber Bragg Grating Sensor for Quantification of DNA

Kavitha, BS and Radhika, NK and Gorthi, SS and Asokan, S (2021) Etched Fiber Bragg Grating Sensor for Quantification of DNA. In: IEEE Sensors Journal, 21 (2). pp. 1588-1595.

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Official URL: https://dx.doi.org/10.1109/JSEN.2020.3020943


The detection and quantification of DNA is of critical importance in various molecular biology processes and has applications in fields ranging from forensics to environmental monitoring. Here, the development of a highly sensitive and label-free technique for DNA detection is reported. This article proposes a reduced graphene oxide (rGO) coated etched Fiber Bragg Grating (eFBG) sensor as a tool for the detection and quantification of dsDNA. The proposed sensor is found to have a limit of detection of 261.87 pg/ μ \textl and the sensor response is a linear function of the dsDNA concentration in the range between 1 ng/ μ \textl to 50 ng/ μ \textl with excellent stability and repeatability. The paper also proposes a technique to quantitatively measure the amount of DNA bound to the sensor and thus, the DNA binding efficacy of the rGO coating on the sensor, utilizing UV spectrometry. © 2001-2012 IEEE.

Item Type: Journal Article
Publication: IEEE Sensors Journal
Publisher: Institute of Electrical and Electronics Engineers Inc.
Additional Information: Copyright to this article belongs to Institute of Electrical and Electronics Engineers Inc.
Keywords: Fiber Bragg gratings; Fiber optic sensors; Graphene; Molecular biology; Reduced Graphene Oxide, Detection and quantifications; Environmental Monitoring; Etched fiber bragg grating; Label-free techniques; Limit of detection; Linear functions; Quantification of dnas; Reduced graphene oxides (RGO), DNA
Department/Centre: Division of Physical & Mathematical Sciences > Instrumentation Appiled Physics
Date Deposited: 18 Jan 2021 09:36
Last Modified: 18 Jan 2021 09:36
URI: http://eprints.iisc.ac.in/id/eprint/67594

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