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Probing the Stepwise Unfolding of Bovine Serum Albumin Using 2D Correlation Raman Spectroscopic Analysis

Kuhar, N and Umapathy, S (2020) Probing the Stepwise Unfolding of Bovine Serum Albumin Using 2D Correlation Raman Spectroscopic Analysis. In: Analytical Chemistry, 92 (19). pp. 13509-13517.

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Official URL: http://dx.doi.org/10.1021/acs.analchem.0c02968


Protein denaturation involves a change in the protein structure with the loss of activity, which proceeds via various intermediates. The possible intermediate structures account largely for understanding the process of unfolding. Hence, considerable attention is required to characterize partially unfolded protein states and to gain more insight into the information about the sequence and steps involved in protein folding mechanisms. In this report, a stepwise unfolding of bovine serum albumin (BSA) with guanidine hydrochloride (GuHCl) has been investigated using Raman spectroscopy in the amide I and III regions. Two-dimensional (2D) correlation analysis has been applied to reveal information on the sequential order and the dynamic properties of interaction during the unfolding process. Raman spectral signatures in the amide I region revealed that there is no significant change in secondary structures up to 2 M concentration of GuHCl. However, 2D correlation analysis further supports the observation by inferring the strengthening of secondary structure at the expense of tertiary structure. At a higher concentration of GuHCl (2-4 M), there is an accumulation of random and β-sheet structures that is mediated by small connecting segments of helices. It further accelerates the unfolding of helices and a complete collapse of structure. These analyses establish the ability of Raman spectroscopy to estimate the ensemble of secondary structures present in proteins. The results reveal the mechanistic details of unfolding, characterizing structure of intermediates even at high concentrations, and understanding the evolution of various secondary structures with respect to each other during unfolding. Such observations can be helpful in understanding the factors affecting the shape and size of proteins during folding/unfolding. Copyright © 2020 American Chemical Society.

Item Type: Journal Article
Publication: Analytical Chemistry
Publisher: American Chemical Society
Additional Information: The copyright of this artile belongs to American Chemical Society
Keywords: Amides; Correlation methods; Mammals; Proteins; Raman spectroscopy, 2D correlation analysis; Bovine serum albumins; Guanidine hydrochloride; Intermediate structures; Protein denaturation; Protein folding mechanisms; Raman spectral signatures; Two Dimensional (2 D), Spectroscopic analysis
Department/Centre: Division of Chemical Sciences > Inorganic & Physical Chemistry
Division of Physical & Mathematical Sciences > Instrumentation Appiled Physics
Date Deposited: 17 Feb 2021 06:46
Last Modified: 23 Feb 2021 10:50
URI: http://eprints.iisc.ac.in/id/eprint/67365

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