Dasgupta, P and Kumar, V and Krishnaswamy, PR and Bhat, N (2020) Biochemical assay for serum creatinine detection through a 1-methylhydantoin and cobalt complex. In: RSC Advances, 10 (64). pp. 39092-39101.
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Abstract
Creatinine is a reliable indicator of renal function and degradation of muscular metabolism. Current analytical techniques for its measurement are limited by their cost and requirement of sophisticated instruments. In this work, we report a highly sensitive amperometric biosensor for creatinine by utilizing the one-step selective conversion of creatinine by creatinine deiminase. The novelty of the proposed sensor relies on the measurement of N-methylhydantoin produced in the reaction. The sensing chemistry comprises of creatinine deiminase as the receptor for creatinine, cobalt chloride as the electrochemically active recognition element, and methylene blue as the redox mediator. The sensing chemistry is immobilized on the glassy carbon electrode surface by physisorption. We have been able to provide a standalone device that reliably quantifies creatinine in serum and even whole blood, without any sample pre-processing. It is possible to measure creatinine in the clinically relevant range from 0.8 to 4 mg dL-1 with this approach. © The Royal Society of Chemistry.
| Item Type: | Journal Article |
|---|---|
| Publication: | RSC Advances |
| Publisher: | Royal Society of Chemistry |
| Additional Information: | The copyright of this article belongs to Royal Society of Chemistry |
| Keywords: | Chlorine compounds; Glass membrane electrodes, Amperometric biosensors; Biochemical assay; Cobalt chlorides; Cobalt complexes; Creatinine detection; Glassy carbon electrodes; Recognition element; Stand-alone devices, Cobalt compounds |
| Department/Centre: | Division of Interdisciplinary Sciences > Centre for Nano Science and Engineering |
| Date Deposited: | 01 Feb 2021 11:45 |
| Last Modified: | 01 Feb 2021 11:45 |
| URI: | http://eprints.iisc.ac.in/id/eprint/67350 |
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