Intramacrophage ROS Primes the Innate Immune System via JAK/STAT and Toll Activation

Chakrabarti, S and Visweswariah, SS (2020) Intramacrophage ROS Primes the Innate Immune System via JAK/STAT and Toll Activation. In: Cell Reports, 33 (6).

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Abstract

Tissue injury is one of the most severe environmental perturbations for a living organism. When damage occurs in adult Drosophila, there is a local response of the injured tissue and a coordinated action across different tissues to help the organism overcome the deleterious effect of an injury. We show a change in the transcriptome of hemocytes at the site of tissue injury, with pronounced activation of the Toll signaling pathway. We find that induction of the cytokine upd-3 and Toll receptor activation occur in response to injury alone, in the absence of a pathogen. Intracellular accumulation of hydrogen peroxide in hemocytes is essential for upd-3 induction and is facilitated by the diffusion of hydrogen peroxide through a channel protein Prip. Importantly, hemocyte activation and production of reactive oxygen species (ROS) at the site of a sterile injury provide protection to flies on subsequent infection, demonstrating training of the innate immune system. © 2020 The Authors Chakrabarti and Visweswariah show that intra-hemocyte ROS is required to activate Toll signaling and induction of upd3 in hemocytes upon injury. Duox is required in hemocytes to increase H2O2 levels, which is facilitated by H2O2 diffusion through a channel protein, Prip. Toll activation protects from subsequent infection with Enterococcus faecali. © 2020 The Authors

Item Type:	Journal Article
Publication:	Cell Reports
Publisher:	Elsevier B.V.
Additional Information:	The copyrights for this article belong to the Author.
Keywords:	aquaporin; cytokine; cytokine upd3; dual oxidase; hydrogen peroxide; Janus kinase; protein Prip; reactive oxygen metabolite; STAT protein; transcriptome; unclassified drug, adult; animal cell; animal experiment; animal model; animal tissue; Article; blood cell; controlled study; Diptera; enterococcal infection; Enterococcus faecalis; innate immunity; macrophage; nonhuman; priority journal; protein expression; tissue injury; TLR signaling; wound infection
Department/Centre:	Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Date Deposited:	23 Dec 2021 10:01
Last Modified:	23 Dec 2021 10:01
URI:	http://eprints.iisc.ac.in/id/eprint/67309

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