Kamat, S and Kumari, M and Sajna, KV and Jayabaskaran, C (2020) Endophytic fungus, Chaetomium globosum, associated with marine green alga, a new source of Chrysin. In: Scientific Reports, 10 (1).
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Abstract
The marine ecosystem is an extraordinary reserve of pharmaceutically important, bioactive compounds even in this �synthetic age�. Marine algae-associated endophytic fungi have gained prominence as an important source of bioactive compounds. This study was conducted on secondary metabolites of Chaetomium globosum-associated with marine green alga Chaetomorpha media from the Konkan coastline, India. Its ethyl acetate extract (CGEE) exhibited an IC50 value of 7.9 ± 0.1 µg/mL on MCF-7 cells. CGEE exhibited G2M phase cell cycle arrest, ROS production and MMP loss in MCF-7 cells. The myco-components in CGEE contributing to the cytotoxicity were found by Gas Chromatography/Mass Spectrometry analyses. Chrysin, a dihydroxyflavone was one of the forty-six myco-components which is commonly found in honey, propolis and passionflower extracts. The compound was isolated and characterized as fungal chrysin using HPLC, UV�Vis spectroscopy, LC�MS, IR and NMR analyses by comparing with standard chrysin. The purified compound exhibited an IC50 value of 49.0 ± 0.6 µM while that of standard chrysin was 48.5 ± 1.6 µM in MCF-7 cells. It induced apoptosis, G1 phase cell cycle arrest, MMP loss, and ROS production. This is the first report of chrysin from an alternative source with opportunities for yield enhancement. © 2020, The Author(s).
Item Type: | Journal Article |
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Publication: | Scientific Reports |
Publisher: | Nature Research |
Additional Information: | The copyright of this article belongs to Nature Research |
Department/Centre: | Division of Biological Sciences > Biochemistry |
Date Deposited: | 15 Jan 2021 09:21 |
Last Modified: | 15 Jan 2021 09:21 |
URI: | http://eprints.iisc.ac.in/id/eprint/67292 |
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