Chaetomium globosum extract mediated gold nanoparticle synthesis and potent anti-inflammatory activity

Ningaraju, S and Munawer, U and Raghavendra, VB and Balaji, KS and Melappa, G and Brindhadevi, K and Pugazhendhi, A (2021) Chaetomium globosum extract mediated gold nanoparticle synthesis and potent anti-inflammatory activity. In: Analytical Biochemistry, 612 .

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Abstract

Gold nanoparticles (AuNPs) are gaining a lot of attention in recent decades from researchers due to their unique optoelectronic properties and their significance in the field of biomedicine. Keeping this in view, our research work was designed to investigate gold nanoparticles obtained by using a fungal endophytic strain Chaetomium globosum, isolated from Vitex negundo which showed significant activity on enzyme inhibition. In the present study, the fungal isolate C. globosum was characterized using HPLC and LC-MS. A novel compound Catechin was matched with standard Catechin. Further, the endophyte C. globosum extract was utilized to synthesize gold nanoparticles (CgAuNPs) which was analysed by UV�visible spectroscopy. The CgAuNPs exhibited wine red color and the absorption peak appeared at 542 nm confirming the formation of the AuNPs. Further, Fourier Transmission Infrared Spectroscopy (FTIR) was performed to confirm the various functional groups present in mycosynthesized CgAuNPs. FTIR analysis demonstrated the presence of amines, flavonoids, as well as the presence of amide I linkage which possibly reduces Au+ to Au0. The synthesized CgAuNPs exhibited potential cytotoxicity against HeLa cells in a dose dependent manner. Further, CgAuNPs demonstrated significant anti-inflammatory activity. Overall, the present work provides insights into the design of nano delivery and may be applied for clinical studies in future. © 2020 Elsevier Inc.

Item Type:	Journal Article
Publication:	Analytical Biochemistry
Publisher:	Academic Press Inc.
Additional Information:	copyright for this article belongs to Academic Press Inc.
Keywords:	Amides; Amines; Enzyme activity; Enzyme inhibition; Fiber optic sensors; Flavonoids; Fourier transform infrared spectroscopy; Gold metallography; Gold nanoparticles; Lanthanum compounds; Metal nanoparticles; Phenols; Synthesis (chemical), Absorption peaks; Anti-inflammatory activity; Chaetomium globosum; Dose-dependent manner; Fungal isolates; Nanoparticle synthesis; Optoelectronic properties; Visible spectroscopy, Gold compounds
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	16 Nov 2020 09:59
Last Modified:	16 Nov 2020 09:59
URI:	http://eprints.iisc.ac.in/id/eprint/66948

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