Phylogenetic conservation of the stem-loop III structure of the 5’untranslated region of Hepatitis C virus RNA among natural variants in samples collected from Southern India

Bhattacharyya, S and Mapa, K and Prabhavathi, S and Sudhamani, SR and Menon, PK and John, KPJ and Shivaram, C and Amarnath, S and Das, S (2004) Phylogenetic conservation of the stem-loop III structure of the 5’untranslated region of Hepatitis C virus RNA among natural variants in samples collected from Southern India. In: Archives of Virology, 149 (5). pp. 1015-1026.

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Abstract

The stem-loop III (SLIII) structure within the 5’untranslated region has been shown to be critical for internal initiation of translation of Hepatitis C virus (HCV). Using ‘Single Strand Conformation Polymorphism (SSCP)’ of the SLIII region we have investigated for natural mutations and demonstrated presence of some non-covariant changes in certain sub-domains. However, overall SLIII-RNA structure was found to be phylogenetically conserved. Additionally, by SSCP analysis we have determined the genotype of 50 HCV isolates collected from Southern India, 25 random samples were confirmed by DNA sequencing. Results showed the prevalence of genotype 1 in this part of India.

Item Type:	Journal Article
Publication:	Archives of Virology
Publisher:	Springer
Additional Information:	The copyright belongs to Springer.
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	15 Nov 2006
Last Modified:	19 Sep 2010 04:26
URI:	http://eprints.iisc.ac.in/id/eprint/6677

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