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Prognosis, diagnosis and the influence of inhibitors: Raman spectroscopic study of radioresistant brain cancer stem-like cells

Kumar, S and Visvanathan, A and Arivazhagan, A and Santhosh, V and Somasundaram, K and Umapathy, S (2020) Prognosis, diagnosis and the influence of inhibitors: Raman spectroscopic study of radioresistant brain cancer stem-like cells. In: Biomedical Spectroscopy, Microscopy, and Imaging 2020, 6-10 April 2020, France.

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Official URL: https://dx.doi.org/10.1117/12.2555381


Glioma Stem-like Cells (GSC) are one of the integral parts of Glioblastoma (GBM) and are generally resistant to standard cancer therapy. Radiation therapy is the standard therapy for GBM which improves the survival of patients and GSCs pose a major obstacle in radiation-induced cell death. Though transcriptomic landscape of different GSCs and their radiation response were well studied, the differences in bio-molecular composition are yet to be explored. Raman microspectroscopy being a non-invasive, label free technique, aids to determine the biomolecular constituent of cells at live conditions. Using GSCs with varying radiation sensitivity (training set), we identified inherent Raman spectral signatures specific to different levels of radiation sensitivity. Moreover, tracking irradiation induced changes in GSCs also identified signatures specific to radiation sensitivity. Both inherent and radiation induced signatures predicted the radiation sensitivity with very high accuracy in the test set. Integrated analysis of inherent and radiation-induced alterations through Raman spectroscopy identified differential regulation of several molecules, in particular glycogen, choline and cholesterol between GSCs with different radio sensitivity. Finally, we reversed the resistant phenotype by using small molecule inhibitors specific for the metabolic pathways of these biomolecules. Thus, we have found Raman spectral signatures to predict radiation sensitivity of GSCs. Further, we also demonstrate radiation-induced molecular changes in the GSCs and methods to reverse radiation sensitivity by using small molecule inhibitors. These findings will have clinical application in radiosensitizing the GSCs. Efficient anti-tumor therapy can be attained with lower dosage of radiation along with these inhibitors with less side-effects. © COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.

Item Type: Conference Paper
Publication: Proceedings of SPIE - The International Society for Optical Engineering
Publisher: SPIE
Additional Information: The copyright of this article belongs to SPIE
Keywords: Antenna lobes; Cell death; Diagnosis; Diseases; Molecules; Radiation effects; Raman spectroscopy; Spectroscopic analysis; Tumors, Differential regulation; Label-free techniques; Radiation sensitivity; Radiation-induced alterations; Raman microspectroscopy; Raman spectral signatures; Raman spectroscopic study; Small molecule inhibitor, Radiotherapy
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Division of Chemical Sciences > Inorganic & Physical Chemistry
Division of Physical & Mathematical Sciences > Instrumentation Appiled Physics
Date Deposited: 02 Mar 2021 06:41
Last Modified: 02 Mar 2021 06:41
URI: http://eprints.iisc.ac.in/id/eprint/66631

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