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NHEJ inhibitor SCR7 and its different forms: Promising CRISPR tools for genome engineering

Ray, U and Vartak, SV and Raghavan, SC (2020) NHEJ inhibitor SCR7 and its different forms: Promising CRISPR tools for genome engineering. In: Gene, 763 .

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Official URL: https://dx.doi.org/10.1016/j.gene.2020.144997


The CRISPR-Cas system currently stands as one of the best multifaceted tools for site-specific genome engineering in mammals. An important aspect of research in this field focusses on improving the specificity and efficacy of precise genome editing in multiple model systems. The cornerstone of this mini-review is one of the extensively investigated small molecule inhibitor, SCR7, which abrogates NHEJ, a Ligase IV-dependent DSB repair pathway, thus guiding integration of the foreign DNA fragment via the more precise homology directed repair during genome editing. One of our recent studies sheds light on properties of different forms of SCR7. Here, we give a succinct account on the use of SCR7 and its different forms in CRISPR-Cas system, highlighting their chemical properties and biological relevance as potent efficiency-enhancing CRISPR tools. © 2020 Elsevier B.V.

Item Type: Journal Article
Publication: Gene
Publisher: Elsevier B.V.
Additional Information: The copyright of this article belongs to Elsevier B.V.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 07 Sep 2020 05:47
Last Modified: 07 Sep 2020 05:47
URI: http://eprints.iisc.ac.in/id/eprint/66468

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