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Changes in T-plastin expression with human trophoblast differentiation

Rao, Rekha M and Rama, S and Rao, Jagannadha A (2003) Changes in T-plastin expression with human trophoblast differentiation. In: Reproductive BioMedicine Online, 7 (2). pp. 235-242.

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During the first trimester of pregnancy, the human placenta is an actively dividing and highly invasive tumour-like tissue, while near term, it represents a fully developed, non-invasive unit. In order to understand the molecular basis of this marked difference in the placental phenotypes, an approach based on a differential display–reverse transcription–polymerase chain reaction (DD–RT–PCR) was adopted to analyse changes in gene expression, using total RNA isolated from first-trimester and term placental villi. Using this approach, T-plastin was initially identified as being differentially expressed in the human first-trimester placenta. T-plastin is an actin-bundling protein and is known to be highly expressed in actively dividing cells and up-regulated in several carcinomas. Using a homogenous population of cytotrophoblasts and syncytiotrophoblasts isolated from human placentae, the present authors demonstrate the differential expression of T-plastin in cytotrophoblasts compared with the terminally differentiated syncytiotrophoblasts. The down-regulation of T-plastin expression is further demonstrated in human trophoblastic BeWo cells induced to differentiate using transforming growth factor $(TGF)^{\beta}$1, a growth factor known for its anti-proliferative and anti-invasive response in placental cells. These studies suggest that expression of T-plastin in the placental context may indeed be associated with the enhanced replicative potential of placental trophoblasts.

Item Type: Journal Article
Publication: Reproductive BioMedicine Online
Publisher: Reproductive Healthcare Ltd
Additional Information: The Copyright belongs to Reproductive Healthcare Ltd.
Keywords: Cytotrophoblast;DD-RT-PCR;Placenta;Proliferation;TGF beta1;T-plastin
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 06 May 2006
Last Modified: 27 Aug 2008 11:57
URI: http://eprints.iisc.ac.in/id/eprint/6621

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